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<p>Smart microscopy is transforming biological imaging by integrating real-time analysis with adaptive acquisition to enhance imaging efficiency. Whereas many emerging implementations are event-driven and focus on on-demand data acquisition to reduce phototoxicity, we here present ‘outcome-driven’ microscopy, a framework combining smart microscopy with optogenetics to control cell biological processes and achieve predefined outcomes. We validate this approach using light-based control of cell migration and nucleocytoplasmic transport, demonstrating robust spatiotemporal control of cellular behaviour in single cells and in cell populations.</p>

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Closed-loop optogenetic control of cell biology enables outcome-driven microscopy

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月23日

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  • Josiah B. Passmore,
  • Alfredo Rates,
  • Jakob Schröder,
  • Menno T. P. van Laarhoven,
  • Vincent J. W. Hellebrekers,
  • Henrik G. van Hoef,
  • Antonius J. M. Geurts,
  • Wendy van Straaten,
  • Wilco Nijenhuis,
  • Florian Berger,
  • Carlas S. Smith,
  • Ihor Smal,
  • Lukas C. Kapitein

摘要

Smart microscopy is transforming biological imaging by integrating real-time analysis with adaptive acquisition to enhance imaging efficiency. Whereas many emerging implementations are event-driven and focus on on-demand data acquisition to reduce phototoxicity, we here present ‘outcome-driven’ microscopy, a framework combining smart microscopy with optogenetics to control cell biological processes and achieve predefined outcomes. We validate this approach using light-based control of cell migration and nucleocytoplasmic transport, demonstrating robust spatiotemporal control of cellular behaviour in single cells and in cell populations.