Scholarlink

<p>Centromeres are essential for kinetochore assembly and spindle attachment. While chromosomes of most species are monocentric with a single centromere, a minority exhibit holocentricity, with a centromere along the chromatid length. Sporadic emergence of holocentricity suggests multiple independent transitions. To explore this, we compare the centromere and (epi)genome organization of two sister genera with contrasting centromere types: <i>Chamaelirium luteum</i> with large macro-monocentromeres and <i>Chionographis japonica</i> with holocentromeres. Both exhibit chromosome-wide histone phosphorylation patterns distinct from typical monocentric species. Kinetochore analysis reveals similar chimeric <i>Borealin</i> in both species, with additional <i>KNL2</i> loss and <i>NSL1</i> chimerism in <i>Cha. luteum</i>. The broad-scale synteny between both genomes supports de novo holocentromere formation in <i>Chi. japonica</i>. Despite sharing features with both centromere types, macro-monocentromeres do not represent a direct link between mono- and holocentromeres. We propose a model for the divergent evolution involving kinetochore gene mutations, altered histone phosphorylation patterns, and centromeric satellite DNA amplification.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Kinetochore mutations and histone phosphorylation pattern changes accompany holo- and macro-monocentromere evolution

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月20日

查看全文
  • Yi-Tzu Kuo,
  • Pavel Neumann,
  • Jianyong Chen,
  • Jörg Fuchs,
  • Veit Schubert,
  • Katrin Kumke,
  • Mariela Analia Sader,
  • Michael Melzer,
  • Zihao Zhu,
  • Axel Himmelbach,
  • Heiko Hentrich,
  • Jiří Macas,
  • Andreas Houben

摘要

Centromeres are essential for kinetochore assembly and spindle attachment. While chromosomes of most species are monocentric with a single centromere, a minority exhibit holocentricity, with a centromere along the chromatid length. Sporadic emergence of holocentricity suggests multiple independent transitions. To explore this, we compare the centromere and (epi)genome organization of two sister genera with contrasting centromere types: Chamaelirium luteum with large macro-monocentromeres and Chionographis japonica with holocentromeres. Both exhibit chromosome-wide histone phosphorylation patterns distinct from typical monocentric species. Kinetochore analysis reveals similar chimeric Borealin in both species, with additional KNL2 loss and NSL1 chimerism in Cha. luteum. The broad-scale synteny between both genomes supports de novo holocentromere formation in Chi. japonica. Despite sharing features with both centromere types, macro-monocentromeres do not represent a direct link between mono- and holocentromeres. We propose a model for the divergent evolution involving kinetochore gene mutations, altered histone phosphorylation patterns, and centromeric satellite DNA amplification.