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<p>We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples. In particular, its effectiveness with low-input cell-free DNA (cfDNA) and hybridization-based target capture highlights its potential for clinical applications, including 5mC biomarker detection and early disease diagnosis.</p>

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Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月13日

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  • Qing Dai,
  • Tanner Baldwin,
  • Ruitu Lyu,
  • Bryan Daniels,
  • Chang Ye,
  • Chen Cao,
  • Chenyou Zhu,
  • Diwen Fan,
  • Liane Lin,
  • Yushuai Liu,
  • Yiding Wang,
  • Chuan He

摘要

We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples. In particular, its effectiveness with low-input cell-free DNA (cfDNA) and hybridization-based target capture highlights its potential for clinical applications, including 5mC biomarker detection and early disease diagnosis.