Scholarlink

<p>Papaya is a fruit crop possessing XY sex chromosomes. The development of long male peduncles (<i>Mp</i>) is pivotal for the transition from gynodioecy to dioecy. The gene controlling peduncle length, <i>CpMp</i>, is one of the four genes driving the evolution of stage 3 sex chromosomes in papaya. Here, we identify a Y-specific SVP paralog, <i>CpSVP-Yp</i>, which is validated as <i>CpMp</i> through comparative genomics and complementation tests. <i>CpMp</i> promotes peduncle elongation by activating <i>CpYUC6</i>, increasing auxin levels, which in turn enhance GA levels and stimulate <i>CpGASA6</i>, driving cell division and elongation. Overexpression of <i>CpGASA6</i> confirms its role and reveals a feedback loop suppressing IAA biosynthesis. We also identify two upstream regulators: <i>CpTRAB1</i>, a GA-repressed activator, and <i>CpGATA8</i>, a <i>CpMp</i>-induced repressor, forming dual feedback circuits. We show that male trees produce 400× more pollen than hermaphrodites, conferring a fitness advantage. Together with two sex determination genes, <i>CpMp</i> is essential for dioecy and sex chromosome evolution in papaya.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification and molecular analysis of the Y-specific CpMp gene controlling long male peduncles in papaya

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月27日

查看全文
  • Yongmei Zhou,
  • Ziqin Pang,
  • Wen Wang,
  • Ray Ming

摘要

Papaya is a fruit crop possessing XY sex chromosomes. The development of long male peduncles (Mp) is pivotal for the transition from gynodioecy to dioecy. The gene controlling peduncle length, CpMp, is one of the four genes driving the evolution of stage 3 sex chromosomes in papaya. Here, we identify a Y-specific SVP paralog, CpSVP-Yp, which is validated as CpMp through comparative genomics and complementation tests. CpMp promotes peduncle elongation by activating CpYUC6, increasing auxin levels, which in turn enhance GA levels and stimulate CpGASA6, driving cell division and elongation. Overexpression of CpGASA6 confirms its role and reveals a feedback loop suppressing IAA biosynthesis. We also identify two upstream regulators: CpTRAB1, a GA-repressed activator, and CpGATA8, a CpMp-induced repressor, forming dual feedback circuits. We show that male trees produce 400× more pollen than hermaphrodites, conferring a fitness advantage. Together with two sex determination genes, CpMp is essential for dioecy and sex chromosome evolution in papaya.