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<p>Soil is the largest organic matter repository on land and the virosphere is an essential component of soil carbon cycling. While a few carbon-related auxiliary metabolic genes (AMGs) in viruses are reported to potentially influence the hosts, the effects of virus-host interactions on soil carbon fixation, particularly in carbon-deficient contaminated soils, need further validation. Here, we explore the impact of viruses on carbon fixation in contaminated soils from 58 metal mining areas across eastern China. Eleven different functional categories of carbon fixation AMGs are identified via metagenomic analysis in 323 contaminated soil samples. Enzymatic activities of three key AMGs (i.e., <i>rbcL</i>, <i>ppdK</i> and <i>TKT</i>) are experimentally characterized, indicating the positive role of these genes in carbon fixation. Furthermore, transcriptomic sequencing reveals that after active virus inoculation the carbon fixation genes significantly up-regulate (~73%, <i>p</i> &lt; 0.05). In mesocosms with stable isotope labeling, the accumulation of <sup>13</sup>C-labeled organic carbon significantly increases (~10%, <i>p</i> &lt; 0.01). Our results provide theoretical and experimental evidence for incorporating viral contributions into the assessments of carbon fixation, and improve the understanding of viral roles within the processes of carbon cycling.</p>

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DNA viral community enhances microbial carbon fixation capacity via auxiliary metabolic genes in contaminated soils

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月13日

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  • Jia-nan Lu,
  • Yuanqing Chao,
  • Li Tian,
  • Xi Zhong,
  • Ziwu Chen,
  • Huan He,
  • Bi Huang,
  • Mengyao Li,
  • Zekai Feng,
  • Huayuan Feng,
  • Chang Hu,
  • Shunkang Zhou,
  • Liqi Zhang,
  • Yulu Yang,
  • Zhepu Ruan,
  • Kengbo Ding,
  • Ying Yang,
  • Ke Yuan,
  • Wenshen Liu,
  • Hua Qi,
  • Yue Cao,
  • Ying-heng Fei,
  • Ning Ling,
  • Shizhong Wang,
  • Ye-tao Tang,
  • Tiangang Luan,
  • Zhihong Xu,
  • Rongliang Qiu

摘要

Soil is the largest organic matter repository on land and the virosphere is an essential component of soil carbon cycling. While a few carbon-related auxiliary metabolic genes (AMGs) in viruses are reported to potentially influence the hosts, the effects of virus-host interactions on soil carbon fixation, particularly in carbon-deficient contaminated soils, need further validation. Here, we explore the impact of viruses on carbon fixation in contaminated soils from 58 metal mining areas across eastern China. Eleven different functional categories of carbon fixation AMGs are identified via metagenomic analysis in 323 contaminated soil samples. Enzymatic activities of three key AMGs (i.e., rbcL, ppdK and TKT) are experimentally characterized, indicating the positive role of these genes in carbon fixation. Furthermore, transcriptomic sequencing reveals that after active virus inoculation the carbon fixation genes significantly up-regulate (~73%, p < 0.05). In mesocosms with stable isotope labeling, the accumulation of 13C-labeled organic carbon significantly increases (~10%, p < 0.01). Our results provide theoretical and experimental evidence for incorporating viral contributions into the assessments of carbon fixation, and improve the understanding of viral roles within the processes of carbon cycling.