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<p>During development, neurons initiate a maturation process during which they start expressing voltage-gated ion channels, form synapses, and start communicating via action potentials. Little is known about external factors regulating this process. Here, we identify environmental mechanics as an important regulator of neuronal maturation, and a molecular pathway linking tissue stiffness to this process. Using patch clamp electrophysiology, calcium imaging and immunofluorescence, we find that, in stiffer environments, neurons show a delay in voltage-gated ion channel activity, action potentials, and synapse formation. RNA sequencing and CRISPR/Cas9 knockdown reveal that the mechanosensitive ion channel Piezo1 supresses transthyretin expression on stiffer substrates, slowing down electrical maturation. In <i>Xenopus laevis</i> embryos, brain stiffness negatively correlates with synapse density, and artificial tissue stiffening delays synaptic activity in vivo. Our data indicate that environmental stiffness represents a fundamental regulator of neuronal maturation, critical for brain circuit development and potentially for neurodevelopmental disorders.</p>

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Environmental stiffness regulates neuronal maturation via Piezo1-mediated transthyretin activity

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月7日

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  • Eva Kreysing,
  • Hélène O. B. Gautier,
  • Sudipta Mukherjee,
  • Katrin A. Mooslehner,
  • Leila Muresan,
  • Daniel Haarhoff,
  • Xiaohui Zhao,
  • Alexander K. Winkel,
  • Tina Borić,
  • Sebastián Vásquez-Sepúlveda,
  • Niklas Gampl,
  • Andrea Dimitracopoulos,
  • Eva K. Pillai,
  • Robert Humphrey,
  • Ragnhildur Thóra Káradóttir,
  • Kristian Franze

摘要

During development, neurons initiate a maturation process during which they start expressing voltage-gated ion channels, form synapses, and start communicating via action potentials. Little is known about external factors regulating this process. Here, we identify environmental mechanics as an important regulator of neuronal maturation, and a molecular pathway linking tissue stiffness to this process. Using patch clamp electrophysiology, calcium imaging and immunofluorescence, we find that, in stiffer environments, neurons show a delay in voltage-gated ion channel activity, action potentials, and synapse formation. RNA sequencing and CRISPR/Cas9 knockdown reveal that the mechanosensitive ion channel Piezo1 supresses transthyretin expression on stiffer substrates, slowing down electrical maturation. In Xenopus laevis embryos, brain stiffness negatively correlates with synapse density, and artificial tissue stiffening delays synaptic activity in vivo. Our data indicate that environmental stiffness represents a fundamental regulator of neuronal maturation, critical for brain circuit development and potentially for neurodevelopmental disorders.