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<p>Influenza virus ribonucleoprotein complexes (RNPs), composed of the polymerase complex (FluPol), nucleoprotein (NP), and RNA, are essential for replication and transcription. We report atomic-resolution cryo-EM structures of mini-vRNPs in two states: FluPol located inside (State-In) or at the outer rim (State-Out) of the NP–RNA ring. In both states, the 5′ and 3′ termini of vRNA are bound to FluPol as previously reported. One NP (NP-0) contacts PA/PB1 of FluPol and binds the distal double-stranded vRNA promoter, with its D72–K90 loop inserting into the RNA fork; separated strands occupy NP-0 RNA-binding grooves. Grooves from other NPs form a continuous RNA-protective path, consistent with negative-strand RNA virus mechanisms. In State-In, interfaces for FluPol dimerization or Pol II interaction are blocked, but fully exposed in State-Out. These structures reveal detailed FluPol–NP–RNA coupling and suggest a conformational shift in RNPs during the viral life cycle.</p>

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Coupling of polymerase-nucleoprotein-RNA in an influenza virus mini ribonucleoprotein complex

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月4日

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  • Huiling Kang,
  • Yunxiang Yang,
  • Yixiao Liu,
  • Mingyu Li,
  • Lejin Zhang,
  • Yuqi Lin,
  • Leander Witte,
  • Kuang-Yu Chen,
  • Wenya Song,
  • Zhili Xu,
  • Xiaojing He,
  • Luke W. Guddat,
  • Yu Guo,
  • Liming Yan,
  • Yan Gao,
  • Ervin Fodor,
  • Zihe Rao,
  • Zhiyong Lou

摘要

Influenza virus ribonucleoprotein complexes (RNPs), composed of the polymerase complex (FluPol), nucleoprotein (NP), and RNA, are essential for replication and transcription. We report atomic-resolution cryo-EM structures of mini-vRNPs in two states: FluPol located inside (State-In) or at the outer rim (State-Out) of the NP–RNA ring. In both states, the 5′ and 3′ termini of vRNA are bound to FluPol as previously reported. One NP (NP-0) contacts PA/PB1 of FluPol and binds the distal double-stranded vRNA promoter, with its D72–K90 loop inserting into the RNA fork; separated strands occupy NP-0 RNA-binding grooves. Grooves from other NPs form a continuous RNA-protective path, consistent with negative-strand RNA virus mechanisms. In State-In, interfaces for FluPol dimerization or Pol II interaction are blocked, but fully exposed in State-Out. These structures reveal detailed FluPol–NP–RNA coupling and suggest a conformational shift in RNPs during the viral life cycle.