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<p>Ewing sarcoma (ES) is an aggressive bone and soft tissue neoplasm characterized by <i>EWSR::ETS</i> rearrangements whose cellular origin remains unclear. EWS::FLI1 expression in human pediatric mesenchymal stem cells (MSCs) induces a transcriptional response distinct from that of human adult MSCs, but fails to form tumors. Here we show that EWS::FLI1 expression in human embryonic mesenchymal stem cells (heMSCs) results in the acquisition of an ES transcriptome, with the oncogene not preferentially binding to gene promoters, but to intronic and intergenic microsatellites. In heMSCs, EWS::FLI1 directly regulates the expression of the DNA repair protein BRCA1, although cells expressing EWS::FLI1 show DNA damage. Xenografting of EWS::FLI1-transduced heMSCs results in the formation of tumors expressing characteristic ES markers. In summary, we show that EWS::FLI1 enforces an aberrant transcriptome and solely is able to endow transforming capacity when expressed in undifferentiated, early heMSCs.</p>

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EWS::FLI1 expression in human embryonic mesenchymal stem cells leads to transcriptional reprograming, defective DNA damage repair and Ewing sarcoma

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月24日

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  • Inmaculada Hernández-Muñoz,
  • Irene Cuervas,
  • Estela Prada,
  • Julian Pulecio,
  • Ramón Gimeno,
  • Evelyn Andrades,
  • Soledad Gómez-González,
  • Pau Berenguer-Molins,
  • Ariadna Acedo-Terrades,
  • Júlia Perera-Bel,
  • Marta Bódalo-Torruella,
  • Lara Nonell,
  • Elena Pérez,
  • Daniela Grases,
  • Caterina Mata,
  • José Yélamos,
  • Yvonne Richaud-Patin,
  • Enrique Vidal,
  • Yasmina Cuartero,
  • François Le Dily,
  • Mariona Suñol,
  • Alejandro Manzanares,
  • Angel Raya,
  • Jaume Mora

摘要

Ewing sarcoma (ES) is an aggressive bone and soft tissue neoplasm characterized by EWSR::ETS rearrangements whose cellular origin remains unclear. EWS::FLI1 expression in human pediatric mesenchymal stem cells (MSCs) induces a transcriptional response distinct from that of human adult MSCs, but fails to form tumors. Here we show that EWS::FLI1 expression in human embryonic mesenchymal stem cells (heMSCs) results in the acquisition of an ES transcriptome, with the oncogene not preferentially binding to gene promoters, but to intronic and intergenic microsatellites. In heMSCs, EWS::FLI1 directly regulates the expression of the DNA repair protein BRCA1, although cells expressing EWS::FLI1 show DNA damage. Xenografting of EWS::FLI1-transduced heMSCs results in the formation of tumors expressing characteristic ES markers. In summary, we show that EWS::FLI1 enforces an aberrant transcriptome and solely is able to endow transforming capacity when expressed in undifferentiated, early heMSCs.