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<p>Leaf rust is a devastating disease of wheat. Growing rust-resistant wheat varieties is the best strategy to mitigate this threat. Here, we generate a 10.51-gigabase chromosome-scale assembly of the durum wheat landrace PI 192051. Using mutagenesis and transcriptome sequencing, we identify the leaf rust resistance gene <i>Lr.ace-4A</i> within a recombination-sparse region of PI 192051 and demonstrate that <i>Lr.ace-4A</i> is identical to the previously designated <i>Lr30</i> gene in hexaploid wheat. <i>Lr.ace-4A</i>/<i>Lr30</i> encodes a non-canonical coiled-coil nucleotide-binding leucine-rich repeat receptor, featuring tandem nucleotide-binding domains. This gene is both necessary and sufficient to confer resistance to leaf rust, as demonstrated by CRISPR/Cas9-induced mutations and transgenic complementation. <i>Lr.ace-4A</i> provides near-immunity resistance in durum wheat, though its effectiveness is diminished in hexaploid wheat. Two amino acid polymorphisms differentiate the resistant and susceptible <i>Lr.ace-4A</i> haplotypes, with transgenic plants carrying either susceptible variant showing susceptibility. The cloning of <i>Lr.ace-4A</i> will accelerate its deployment in wheat breeding programs.</p>

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Genome-assisted identification of wheat leaf rust resistance gene Lr.ace-4A/Lr30

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月22日

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  • Jinwei Yang,
  • Hongna Li,
  • Mengyu Li,
  • Rui Song,
  • Tao Shen,
  • Guiping Wang,
  • Dong Xu,
  • Ming Hao,
  • Aolin Jia,
  • Shams ur Rehman,
  • Lei Hua,
  • Yanyan Liang,
  • Cheng Chi,
  • Caixia Lan,
  • Xing Wang Deng,
  • Jorge Dubcovsky,
  • Baoxing Song,
  • Xiaodong Wang,
  • Shisheng Chen

摘要

Leaf rust is a devastating disease of wheat. Growing rust-resistant wheat varieties is the best strategy to mitigate this threat. Here, we generate a 10.51-gigabase chromosome-scale assembly of the durum wheat landrace PI 192051. Using mutagenesis and transcriptome sequencing, we identify the leaf rust resistance gene Lr.ace-4A within a recombination-sparse region of PI 192051 and demonstrate that Lr.ace-4A is identical to the previously designated Lr30 gene in hexaploid wheat. Lr.ace-4A/Lr30 encodes a non-canonical coiled-coil nucleotide-binding leucine-rich repeat receptor, featuring tandem nucleotide-binding domains. This gene is both necessary and sufficient to confer resistance to leaf rust, as demonstrated by CRISPR/Cas9-induced mutations and transgenic complementation. Lr.ace-4A provides near-immunity resistance in durum wheat, though its effectiveness is diminished in hexaploid wheat. Two amino acid polymorphisms differentiate the resistant and susceptible Lr.ace-4A haplotypes, with transgenic plants carrying either susceptible variant showing susceptibility. The cloning of Lr.ace-4A will accelerate its deployment in wheat breeding programs.