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<p>Wheat has evolved diverse resistance genes against powdery mildew, typically controlled by single-gene-encoded proteins. Here, we report the map-based cloning of <i>PmWR183</i>, a resistance locus encoding two adjacent NLR proteins (<i>PmWR183-NLR1</i> and <i>PmWR183-NLR2</i>) from wild emmer wheat. Stable transformation and CRISPR/Cas9 knockout experiments demonstrate that the two NLRs function cooperatively: neither gene alone confers resistance, but their co-expression restores immunity, while disruption of either gene abolishes resistance. <i>PmWR183</i> mediates a developmental stage-dependent response, with susceptibility at the seedling stage and strong resistance at the adult stage. Protein interaction assays reveal constitutive association of PmWR183-NLR1 and PmWR183-NLR2, supporting their cooperative role. Geographical and haplotype analyses show the locus originates from wild emmer and is rare in cultivated wheat, exhibiting at least nine haplotypes. Together, our findings uncover a rare NLR gene pair conferring effective resistance to powdery mildew, providing valuable resources for wheat breeding.</p>

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Paired NLRs originated from Triticum dicoccoides coordinately confer resistance to powdery mildew in wheat

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月10日

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  • Huaizhi Zhang,
  • Miaomiao Li,
  • Gaojie Wang,
  • Keyu Zhu,
  • Guanghao Guo,
  • Hongkui Fu,
  • Chenchen Hu,
  • Zhiying Chu,
  • Jinghuang Hu,
  • Qiuhong Wu,
  • Yongxing Chen,
  • Dan Qiu,
  • Jingzhong Xie,
  • Delin Li,
  • Beibei Li,
  • Wenling Li,
  • Lei Dong,
  • Yikun Hou,
  • Xuejia Cui,
  • Baoge Huang,
  • Yi Liu,
  • Yiwen Li,
  • Hongjie Li,
  • Chengguo Yuan,
  • Lingli Dong,
  • Zhiyong Liu,
  • Ping Lu

摘要

Wheat has evolved diverse resistance genes against powdery mildew, typically controlled by single-gene-encoded proteins. Here, we report the map-based cloning of PmWR183, a resistance locus encoding two adjacent NLR proteins (PmWR183-NLR1 and PmWR183-NLR2) from wild emmer wheat. Stable transformation and CRISPR/Cas9 knockout experiments demonstrate that the two NLRs function cooperatively: neither gene alone confers resistance, but their co-expression restores immunity, while disruption of either gene abolishes resistance. PmWR183 mediates a developmental stage-dependent response, with susceptibility at the seedling stage and strong resistance at the adult stage. Protein interaction assays reveal constitutive association of PmWR183-NLR1 and PmWR183-NLR2, supporting their cooperative role. Geographical and haplotype analyses show the locus originates from wild emmer and is rare in cultivated wheat, exhibiting at least nine haplotypes. Together, our findings uncover a rare NLR gene pair conferring effective resistance to powdery mildew, providing valuable resources for wheat breeding.