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<p>Microautophagy is an intracellular degradation process in which degradatory organelles, such as the lysosome, directly take up substrates by invagination and/or protrusion of their membranes. Here, we provide evidence that Rab32-positive, lysosome-related organelles in macrophages incorporate various other organelles, including endosomes and mitochondria. Our data indicates that, upon exposure to a mitochondria-damaging reagent, mitochondria can be directly engulfed by the lysosome-like organelles independently of macroautophagy or ESCRT machinery. Rab32 GTPase, phosphatidylinositol 3,5-bisphosphates, ubiquitination, and p62/SQSTM1 are crucial for this degradation. Furthermore, the degree of M1 polarization of macrophages, which is facilitated by metabolic reprogramming into increased glycolysis via mitochondrial elimination, is significantly reduced in <i>Rab32/38</i> double-knockout macrophages. Thus, microautophagy plays a role in the physiological regulation of macrophages.</p>

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Evidence that mitochondria in macrophages are destroyed by microautophagy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月30日

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  • Shiou-Ling Lu,
  • Siyu Chen,
  • Kazuya Noda,
  • Yangjie Li,
  • Chao-Yuan Tsai,
  • Hiroko Omori,
  • Yumiko Kato,
  • Zidi Zhang,
  • Bohan Chen,
  • Kanako Tokuda,
  • Tongxin Zheng,
  • Masahiro Wakita,
  • Eiji Hara,
  • Mitsunori Fukuda,
  • Yoh Wada,
  • Eiji Morita,
  • Narikazu Uzawa,
  • Shinya Murakami,
  • Takeshi Noda

摘要

Microautophagy is an intracellular degradation process in which degradatory organelles, such as the lysosome, directly take up substrates by invagination and/or protrusion of their membranes. Here, we provide evidence that Rab32-positive, lysosome-related organelles in macrophages incorporate various other organelles, including endosomes and mitochondria. Our data indicates that, upon exposure to a mitochondria-damaging reagent, mitochondria can be directly engulfed by the lysosome-like organelles independently of macroautophagy or ESCRT machinery. Rab32 GTPase, phosphatidylinositol 3,5-bisphosphates, ubiquitination, and p62/SQSTM1 are crucial for this degradation. Furthermore, the degree of M1 polarization of macrophages, which is facilitated by metabolic reprogramming into increased glycolysis via mitochondrial elimination, is significantly reduced in Rab32/38 double-knockout macrophages. Thus, microautophagy plays a role in the physiological regulation of macrophages.