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<p>Scalable and high-throughput platforms to non-invasively record the Action Potentials (APs) of excitable cells are highly demanded to accelerate disease diagnosis and drug discovery. AP recordings are typically achieved with the invasive and low-throughput patch clamp technique. Non-invasive alternatives like planar multielectrode arrays cannot record APs without membrane poration, preventing accurate measurements of disease states and drug effects. Here, we disclose reliable and non-invasive recording of APs with patch clamp-like quality from human stem cell-derived cardiomyocytes using an inkjet-printed polymer semiconductor in an Electrolyte-Gated Field-Effect Transistor configuration. High sensitivity is proven by the detection of drug-induced pro-arrhythmic membrane potential oscillations as early/delayed afterdepolarizations. The higher throughput potential of this platform could significantly enhance disease modelling, drug screening, safety pharmacology and the study of abiotic/biotic interfaces.</p>

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Non-invasive action potential recordings using printed electrolyte-gated polymer field-effect transistors

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月31日

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  • Adrica Kyndiah,
  • Giulia Zoe Zemignani,
  • Carlotta Ronchi,
  • Gabriele Tullii,
  • Aleksandr Khudiakov,
  • Giuseppina Iachetta,
  • Stefano Chiodini,
  • Rosalia Moreddu,
  • Fabrizio Antonio Viola,
  • Peter J. Schwartz,
  • Gabriel Gomila,
  • Francesco De Angelis,
  • Luca Sala,
  • Maria Rosa Antognazza,
  • Mario Caironi

摘要

Scalable and high-throughput platforms to non-invasively record the Action Potentials (APs) of excitable cells are highly demanded to accelerate disease diagnosis and drug discovery. AP recordings are typically achieved with the invasive and low-throughput patch clamp technique. Non-invasive alternatives like planar multielectrode arrays cannot record APs without membrane poration, preventing accurate measurements of disease states and drug effects. Here, we disclose reliable and non-invasive recording of APs with patch clamp-like quality from human stem cell-derived cardiomyocytes using an inkjet-printed polymer semiconductor in an Electrolyte-Gated Field-Effect Transistor configuration. High sensitivity is proven by the detection of drug-induced pro-arrhythmic membrane potential oscillations as early/delayed afterdepolarizations. The higher throughput potential of this platform could significantly enhance disease modelling, drug screening, safety pharmacology and the study of abiotic/biotic interfaces.