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<p><i>Wolbachia</i> is a promising strategy to inhibit dengue virus (DENV) transmission by <i>Ae. aegypti</i> mosquitoes. Laboratory studies assessing DENV inhibition by <i>Wolbachia</i> typically have not considered natural frequent mosquito blood feeding behavior. Here, we determine the impact of successive feeding on DENV-2 transmission by <i>Ae. aegypti</i> in the presence or absence of <i>Wolbachia</i> (<i>w</i>AlbB and <i>w</i>MelM strains). We show that successive feeding shortens the extrinsic incubation period (EIP) in wildtype (WT; without <i>Wolbachia</i>) and <i>w</i>AlbB mosquitoes through enhanced dissemination. Feeding empirical data into models showed that successive feeding increases the probability of WT and <i>w</i>AlbB mosquitoes surviving beyond the EIP. Importantly, the more epidemiologically relevant comparison of the odds of <i>w</i>AlbB mosquitoes surviving beyond the EIP relative to WT, reveals a larger impact of successive feeding on WT than <i>w</i>AlbB. This indicates a strong inhibitory effect of <i>Wolbachia</i> even in the context of natural frequent mosquito blood feeding behavior.</p>

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Implications of successive blood feeding on Wolbachia-mediated dengue virus inhibition in Aedes aegypti mosquitoes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月29日

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  • Rebecca M. Johnson,
  • Mallery I. Breban,
  • Braiya L. Nolan,
  • Afeez Sodeinde,
  • Isabel M. Ott,
  • Perran A. Ross,
  • Xinyue Gu,
  • Nathan D. Grubaugh,
  • T. Alex Perkins,
  • Doug E. Brackney,
  • Chantal B. F. Vogels

摘要

Wolbachia is a promising strategy to inhibit dengue virus (DENV) transmission by Ae. aegypti mosquitoes. Laboratory studies assessing DENV inhibition by Wolbachia typically have not considered natural frequent mosquito blood feeding behavior. Here, we determine the impact of successive feeding on DENV-2 transmission by Ae. aegypti in the presence or absence of Wolbachia (wAlbB and wMelM strains). We show that successive feeding shortens the extrinsic incubation period (EIP) in wildtype (WT; without Wolbachia) and wAlbB mosquitoes through enhanced dissemination. Feeding empirical data into models showed that successive feeding increases the probability of WT and wAlbB mosquitoes surviving beyond the EIP. Importantly, the more epidemiologically relevant comparison of the odds of wAlbB mosquitoes surviving beyond the EIP relative to WT, reveals a larger impact of successive feeding on WT than wAlbB. This indicates a strong inhibitory effect of Wolbachia even in the context of natural frequent mosquito blood feeding behavior.