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<p>(+)-<i>cis</i>-12-oxo-phytodienoic acid (<i>cis</i>-OPDA) is a biosynthetic precursor of the plant hormone (+)−7-<i>iso</i>-Jasmonoyl-L-isoleucine (JA-Ile). It was proposed as an endogenous chemical signal independent of the JA-Ile receptor COI1-JAZ in <i>Arabidopsis thaliana</i>. The bioactive form of <i>cis</i>-OPDA that induces COI1-JAZ-independent gene expression remains unknown. Here we demonstrate that the downstream metabolites of <i>cis</i>-OPDA, especially tetranor-<i>cis</i>-OPDA (tn-<i>cis</i>-OPDA) and 7-<i>iso</i>-4,5-didehydro-JA (4,5-ddh-JA), upregulate the expression of the OPDA marker genes such as <i>ZAT10</i> and <i>ERF5</i>. These downstream metabolites function independently of the JA-Ile-COI1-JAZ-MYCs canonical jasmonate signaling module, and their electrophilic α, β-unsaturated carbonyl functionalities are essential for their bioactivity. These results raise a possibility that the downstream metabolites of <i>cis</i>-OPDA would function as endogenous chemical signal in <i>A. thaliana</i>.</p>

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Downstream metabolites of (+)-cis-12-oxo-phytodienoic acid function as noncanonical bioactive jasmonates in Arabidopsis thaliana

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月21日

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  • Minoru Ueda,
  • Rina Saito,
  • Yuho Nishizato,
  • Tsumugi Kitajima,
  • Nobuki Kato

摘要

(+)-cis-12-oxo-phytodienoic acid (cis-OPDA) is a biosynthetic precursor of the plant hormone (+)−7-iso-Jasmonoyl-L-isoleucine (JA-Ile). It was proposed as an endogenous chemical signal independent of the JA-Ile receptor COI1-JAZ in Arabidopsis thaliana. The bioactive form of cis-OPDA that induces COI1-JAZ-independent gene expression remains unknown. Here we demonstrate that the downstream metabolites of cis-OPDA, especially tetranor-cis-OPDA (tn-cis-OPDA) and 7-iso-4,5-didehydro-JA (4,5-ddh-JA), upregulate the expression of the OPDA marker genes such as ZAT10 and ERF5. These downstream metabolites function independently of the JA-Ile-COI1-JAZ-MYCs canonical jasmonate signaling module, and their electrophilic α, β-unsaturated carbonyl functionalities are essential for their bioactivity. These results raise a possibility that the downstream metabolites of cis-OPDA would function as endogenous chemical signal in A. thaliana.