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<p>Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an <i>Opisthorchis viverrini</i> recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of <i>O. viverrini</i> infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.</p>

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Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Lakkhana Sadaow,
  • Rutchanee Rodpai,
  • Michael J. Smout,
  • Rie Nakajima,
  • Patcharaporn Boonroumkaew,
  • Javier Sotillo,
  • Bemnet A. Tedla,
  • Vor Luvira,
  • Amnat Kitkhuandee,
  • Krisada Paonariang,
  • Wattana Sukeepaisarnjaroen,
  • Hiroshi Yamasaki,
  • Sutas Suttiprapa,
  • Thewarach Laha,
  • Banchob Sripa,
  • Rafael de Assis,
  • Aarti Jain,
  • Wannaporn Ittiprasert,
  • Victoria H. Mann,
  • Yide Wong,
  • Philip L. Felgner,
  • Wanchai Maleewong,
  • Paul J. Brindley,
  • Alex Loukas,
  • Pewpan M. Intapan

摘要

Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.