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<p><i>Rehmannia chingii</i> (2n = 2x = 28) is an important folk medicinal plant with high therapeutic value, particularly due to its richness in iridoid glycosides. However, research on its evolution and gene functional identification has been hindered by the lack of a high-quality genome. Here, we present the 1.169 Gb telomere-to-telomere (T2T) genome sequence of <i>R. chingii</i>. Phylogenetic analysis confirms that <i>Rehmannia</i> belongs to the Orobanchaceae family. We find that structural genes of the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway and the iridoid pathway are predominantly expressed in <i>R. chingii</i> leaves. Further analyses reveal a cytochrome P450 gene cluster localized on chromosome 8, and identify RcCYP72H7 within this cluster as an aucubin epoxidase, capable of catalyzing aucubin epoxidation to form catalpol. The genome offers valuable resources for studying iridoid glycoside biosynthesis and the evolutionary history of <i>Rehmannia</i>, and will help to faciliate genetic improvement of <i>R. chingii</i> for pharmaceutical and health-related applications.</p>

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Analysis of the Rehmannia chingii genome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Fengqing Wang,
  • Zhennan Jiang,
  • Junge Gao,
  • Chunyan Miao,
  • Ci Song,
  • Yahe Yang,
  • Ning Ding,
  • Yajing Li,
  • Hongzheng Sun,
  • Caixia Xie,
  • Bao Zhang,
  • Pengyu Zhang,
  • Li Gu,
  • Qiuwen Hao,
  • Zhongyi Zhang,
  • Luqi Huang,
  • Yuan Yuan

摘要

Rehmannia chingii (2n = 2x = 28) is an important folk medicinal plant with high therapeutic value, particularly due to its richness in iridoid glycosides. However, research on its evolution and gene functional identification has been hindered by the lack of a high-quality genome. Here, we present the 1.169 Gb telomere-to-telomere (T2T) genome sequence of R. chingii. Phylogenetic analysis confirms that Rehmannia belongs to the Orobanchaceae family. We find that structural genes of the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway and the iridoid pathway are predominantly expressed in R. chingii leaves. Further analyses reveal a cytochrome P450 gene cluster localized on chromosome 8, and identify RcCYP72H7 within this cluster as an aucubin epoxidase, capable of catalyzing aucubin epoxidation to form catalpol. The genome offers valuable resources for studying iridoid glycoside biosynthesis and the evolutionary history of Rehmannia, and will help to faciliate genetic improvement of R. chingii for pharmaceutical and health-related applications.