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<p>In eukaryotic cells, reactive oxygen species (ROS) serve as crucial signaling components. ROS are potentially toxic, so constant adjustments are needed to maintain cellular health. Here we describe a single-cell, mass cytometry-based method that we call signaling network under redox stress profiling (SN-ROP) to monitor dynamic changes in redox-related pathways during redox stress. SN-ROP quantifies ROS transporters, enzymes, oxidative stress products and associated signaling pathways to provide information on cellular redox regulation. Applied to diverse cell types and conditions, SN-ROP reveals unique redox patterns and dynamics including coordinated shifts in CD8<sup>+</sup> T cells upon antigen stimulation as well as variations in CAR-T cell persistence. Furthermore, SN-ROP analysis uncovers environmental factors such as hypoxia and T cell exhaustion for influencing redox balance, and also reveals distinct features in patients on hemodialysis. Our findings thus support the use of SN-ROP to elucidate intricate redox networks and their implications in immune cell function and disease.</p>

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Single-cell signaling network profiling during redox stress reveals dynamic redox regulation in immune cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Yi-Chuan Wang,
  • Ping-Hsun Wu,
  • Wen-Chieh Ting,
  • Yi-Fu Wang,
  • Ming-Han Yang,
  • Tung-Hung Su,
  • Jia-Ying Su,
  • Hsun-I Sun,
  • Wei-Min Huang,
  • Pei-Ling Tsai,
  • Gerlinde Wernig,
  • Ping-Chih Ho,
  • Limei Wang,
  • Chen-Tu Wu,
  • Yih-Leong Chang,
  • Tseng-Cheng Chen,
  • Tzu-Ching Meng,
  • Yao-Ming Chang,
  • Shih-Lei Lai,
  • Chia-Wei Li,
  • Tai-Ming Ko,
  • Kai-Chien Yang,
  • Ya-Jen Chang,
  • Yijuang Chern,
  • Mei-Chuan Kuo,
  • Yen-Tsung Huang,
  • Yi-Shiuan Tzeng,
  • Jih-Luh Tang,
  • Shih-Yu Chen

摘要

In eukaryotic cells, reactive oxygen species (ROS) serve as crucial signaling components. ROS are potentially toxic, so constant adjustments are needed to maintain cellular health. Here we describe a single-cell, mass cytometry-based method that we call signaling network under redox stress profiling (SN-ROP) to monitor dynamic changes in redox-related pathways during redox stress. SN-ROP quantifies ROS transporters, enzymes, oxidative stress products and associated signaling pathways to provide information on cellular redox regulation. Applied to diverse cell types and conditions, SN-ROP reveals unique redox patterns and dynamics including coordinated shifts in CD8+ T cells upon antigen stimulation as well as variations in CAR-T cell persistence. Furthermore, SN-ROP analysis uncovers environmental factors such as hypoxia and T cell exhaustion for influencing redox balance, and also reveals distinct features in patients on hemodialysis. Our findings thus support the use of SN-ROP to elucidate intricate redox networks and their implications in immune cell function and disease.