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<p>Two circular chromosomes are a defining feature of the bacterial family <i>Vibrionaceae</i>, including the pathogen <i>Vibrio cholerae</i>, with rare reports of isolates with a single, fused chromosome. Here, we use long-read sequencing to analyse 467 <i>V. cholerae</i> O1 isolates from 47 cholera patients and household contacts in Bangladesh. We identify several independent chromosome fusion events that are likely transmissible within a household. Fusions occur in a 12 kilobase-pair homologous sequence shared between the two chromosomes and are stable for&#xa0;at least 200 generations under laboratory conditions. We find no detectable effect of fusion on <i>V. cholerae</i> growth, virulence factor expression, or biofilm formation. The factors promoting fusion, affecting chromosome stability, and subtle phenotypic or clinical consequences merit further investigation.</p>

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Prevalent chromosome fusion in Vibrio cholerae O1

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Aline Cuénod,
  • Denise Chac,
  • Ashraful I. Khan,
  • Fahima Chowdhury,
  • Randy W. Hyppa,
  • Susan M. Markiewicz,
  • Amelia Rice,
  • Akhil Kholwadwala,
  • Stephen B. Calderwood,
  • Edward T. Ryan,
  • Jason B. Harris,
  • Regina C. LaRocque,
  • Taufiqur R. Bhuiyan,
  • Gerald R. Smith,
  • Firdausi Qadri,
  • Patrick Lypaczewski,
  • Ana A. Weil,
  • B. Jesse Shapiro

摘要

Two circular chromosomes are a defining feature of the bacterial family Vibrionaceae, including the pathogen Vibrio cholerae, with rare reports of isolates with a single, fused chromosome. Here, we use long-read sequencing to analyse 467 V. cholerae O1 isolates from 47 cholera patients and household contacts in Bangladesh. We identify several independent chromosome fusion events that are likely transmissible within a household. Fusions occur in a 12 kilobase-pair homologous sequence shared between the two chromosomes and are stable for at least 200 generations under laboratory conditions. We find no detectable effect of fusion on V. cholerae growth, virulence factor expression, or biofilm formation. The factors promoting fusion, affecting chromosome stability, and subtle phenotypic or clinical consequences merit further investigation.