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<p>With the increasing challenges posed by global warming and climate change, heat stress has become a significant threat to the sustainable production of grapevines. However, the genetic basis of grapevine thermotolerance remains poorly understood. Here, we combine genome-wide association study with transcriptomic profiling to identify <i>TTC4</i> (<i>thermotolerance on chromosome 4</i>), a gene encoding a WRKY transcription factor, as a key determinant of thermotolerance in grapevine. TTC4 directly activates two thermotolerance-related genes, <i>HSP18.1</i> and <i>APX3</i>. We also identify a heat-suppressed repressor SPL13 (SQUAMOSA-promoter binding protein-like 13) that cannot bind to the GTAT element (<i>TTC4</i><sup>T(7631)</sup>) in intron 2 of <i>TTC4</i>, but can bind to the natural variant, GTAC (<i>TTC4</i><sup>C(7631)</sup>). Grapevine accessions with <i>TTC4</i><sup>C/C(7631)</sup> genotype exhibit significantly lower thermotolerance compared to those with the <i>TTC4</i><sup>T/T(7631)</sup> and <i>TTC4</i><sup>C/T(7631)</sup> genotypes. This fine-tuned regulation contributes to thermotolerance divergence among grapevine populations. The <i>TTC4</i><sup>T(7631)</sup> haplotype holds significant potential as a genetic resource for breeding thermotolerant grapevine varieties.</p>

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A naturally occurring SNP modulates thermotolerance divergence among grapevines

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月1日

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  • Haiyang Chen,
  • Haibo Yu,
  • Ling Yuan,
  • Lingchao Kong,
  • Shenchang Li,
  • Xiongjun Cao,
  • Yang Li,
  • Yi Wang,
  • Ling Lin,
  • Rongrong Guo,
  • Taili Xie,
  • Wei Duan,
  • Zhanwu Dai,
  • Peige Fan,
  • Shaohua Li,
  • Zhenchang Liang,
  • Lijun Wang

摘要

With the increasing challenges posed by global warming and climate change, heat stress has become a significant threat to the sustainable production of grapevines. However, the genetic basis of grapevine thermotolerance remains poorly understood. Here, we combine genome-wide association study with transcriptomic profiling to identify TTC4 (thermotolerance on chromosome 4), a gene encoding a WRKY transcription factor, as a key determinant of thermotolerance in grapevine. TTC4 directly activates two thermotolerance-related genes, HSP18.1 and APX3. We also identify a heat-suppressed repressor SPL13 (SQUAMOSA-promoter binding protein-like 13) that cannot bind to the GTAT element (TTC4T(7631)) in intron 2 of TTC4, but can bind to the natural variant, GTAC (TTC4C(7631)). Grapevine accessions with TTC4C/C(7631) genotype exhibit significantly lower thermotolerance compared to those with the TTC4T/T(7631) and TTC4C/T(7631) genotypes. This fine-tuned regulation contributes to thermotolerance divergence among grapevine populations. The TTC4T(7631) haplotype holds significant potential as a genetic resource for breeding thermotolerant grapevine varieties.