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<p>The CEBPA transcription factor is frequently mutated in acute myeloid leukemia (AML). Mutations in the <i>CEBPA</i> gene, which are typically biallelic, result in the production of a shorter isoform known as p30. Both the canonical 42-kDa isoform (p42) and the AML-associated p30 isoform bind chromatin and activate transcription, but the specific transcriptional programs controlled by each protein and how they are linked to a selective advantage in AML is not well understood. Here, we show that cells expressing the AML-associated p30 have reduced baseline inflammatory gene expression and display altered dynamics of transcriptional induction in response to LPS, consequently impacting cytokine secretion. This confers p30-expressing cells an increased resistance to the adverse effects of prolonged exposure to inflammatory signals. Mechanistically, we show that these differences primarily arise from the differential regulation of AP-1 family proteins. In addition, we find that the impaired function of the AP-1 member ATF4 in p30-expressing cells alters their response to ER stress. Collectively, these findings uncover a link between mutant CEBPA, inflammation and the stress response, potentially revealing a vulnerability in AML.</p>

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Mutant CEBPA promotes tolerance to inflammatory stress through deficient AP-1 activation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月12日

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  • Maria Cadefau-Fabregat,
  • Gerard Martínez-Cebrián,
  • Lucía Lorenzi,
  • Felix D. Weiss,
  • Anne-Katrine Frank,
  • José Manuel Castelló-García,
  • Eric Julià-Vilella,
  • Andrés Gámez-García,
  • Laura Yera,
  • Carini Picardi Morais de Castro,
  • Yi-Fang Wang,
  • Felix Meissner,
  • Alejandro Vaquero,
  • Matthias Merkenschlager,
  • Bo T. Porse,
  • Sergi Cuartero

摘要

The CEBPA transcription factor is frequently mutated in acute myeloid leukemia (AML). Mutations in the CEBPA gene, which are typically biallelic, result in the production of a shorter isoform known as p30. Both the canonical 42-kDa isoform (p42) and the AML-associated p30 isoform bind chromatin and activate transcription, but the specific transcriptional programs controlled by each protein and how they are linked to a selective advantage in AML is not well understood. Here, we show that cells expressing the AML-associated p30 have reduced baseline inflammatory gene expression and display altered dynamics of transcriptional induction in response to LPS, consequently impacting cytokine secretion. This confers p30-expressing cells an increased resistance to the adverse effects of prolonged exposure to inflammatory signals. Mechanistically, we show that these differences primarily arise from the differential regulation of AP-1 family proteins. In addition, we find that the impaired function of the AP-1 member ATF4 in p30-expressing cells alters their response to ER stress. Collectively, these findings uncover a link between mutant CEBPA, inflammation and the stress response, potentially revealing a vulnerability in AML.