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<p>Competition among bacteria for carbohydrates is pivotal for colonization resistance (CR). However, the impact of Western-style diets on CR remains unclear. Here we show how the competition between <i>Klebsiella oxytoca</i> and <i>Klebsiella pneumoniae</i> is modulated by consuming one of three Western-style diets characterized by high-starch, high-sucrose, or high-fat/high-sucrose content. In vivo competition experiments in ampicillin-treated mice reveal that <i>K. oxytoca</i> promotes <i>K. pneumoniae</i> decolonization on all dietary backgrounds. However, mice on the high-fat/high-sucrose diet show reduced pathogen clearance. Microbiome analysis reveals that the combination of Western-style diets and ampicillin treatment synergize in microbiome impairment, particularly noticeable in the presence of high dietary fat content. The diet-independent degradation of ampicillin in the gut lumen by <i>K. oxytoca</i> beta-lactamases facilitates rapid commensal outgrowth, which is required for subsequent pathogen clearance. Our findings provide insights into how diet modulates functional microbiome recovery and <i>K. oxytoca</i>-mediated pathogen elimination from the gut.</p>

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Klebsiella oxytoca facilitates microbiome recovery via antibiotic degradation and restores colonization resistance in a diet-dependent manner

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月9日

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  • Éva d. H. Almási,
  • Lea Eisenhard,
  • Lisa Osbelt,
  • Till Robin Lesker,
  • Anna C. Vetter,
  • Nele Knischewski,
  • Agata Anna Bielecka,
  • Achim Gronow,
  • Uthayakumar Muthukumarasamy,
  • Marie Wende,
  • Caroline Tawk,
  • Meina Neumann-Schaal,
  • Mark Brönstrup,
  • Till Strowig

摘要

Competition among bacteria for carbohydrates is pivotal for colonization resistance (CR). However, the impact of Western-style diets on CR remains unclear. Here we show how the competition between Klebsiella oxytoca and Klebsiella pneumoniae is modulated by consuming one of three Western-style diets characterized by high-starch, high-sucrose, or high-fat/high-sucrose content. In vivo competition experiments in ampicillin-treated mice reveal that K. oxytoca promotes K. pneumoniae decolonization on all dietary backgrounds. However, mice on the high-fat/high-sucrose diet show reduced pathogen clearance. Microbiome analysis reveals that the combination of Western-style diets and ampicillin treatment synergize in microbiome impairment, particularly noticeable in the presence of high dietary fat content. The diet-independent degradation of ampicillin in the gut lumen by K. oxytoca beta-lactamases facilitates rapid commensal outgrowth, which is required for subsequent pathogen clearance. Our findings provide insights into how diet modulates functional microbiome recovery and K. oxytoca-mediated pathogen elimination from the gut.