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<p>A novel culturable insect-derived actinobacterium, designated strain Odt1-20B<sup>T</sup>, was isolated from <i>Odontotermes formosanus</i>. The cells exhibited aerobic, Gram-stain-positive, and filamentous characteristics. Strain Odt1-20B<sup>T</sup> formed well-developed substrate and aerial mycelia, with straight spore chains borne on the aerial mycelium. Strain Odt1-20B<sup>T</sup> grew at 20–40 °C (optimum 30 °C), 0˗4% (w/v) NaCl, and pH 5˗10 (optimum 7−8). The <span>l</span>,<span>l</span>-diaminopimelic acid was determined in the whole-cell hydrolysate of strain Odt1-20B<sup>T</sup>. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine, whereas the detected major quinones were MK-9(H<sub>4</sub>), MK-9(H<sub>6</sub>), and MK-9(H<sub>8</sub>). Whole-cell sugars were galactose, glucose, mannose, ribose, and xylose. Summed feature C<sub>18:1</sub> ω6c/ω7c, C<sub>16:0</sub>, <i>anteiso</i>-C<sub>15:0</sub>, and <i>iso</i>-C<sub>16:0</sub> were the main fatty acids. The draft genome size was 10,091,787 nucleotides, with a G + C content of 71.5%. Comparative phylogenetic analysis based on 16S rRNA gene sequence demonstrated that strain Odt1-20B<sup>T</sup> belonged to the genus <i>Streptomyces</i>, and shared the highest similarity with <i>S. davaonensis</i> JCM 4913 <sup>T</sup> (98.55%). Furthermore, phylogenomic analysis showed that strain Odt1-20B<sup>T</sup> was clearly distinguished from the closely related <i>Streptomyces</i> species. The average nucleotide identity analyses based on BLAST (ANIb) and MUMmer (ANIm) between strain Odt1-20B<sup>T</sup> and its related species ranged below 87%, and the digital DNA-DNA hybridization (dDDH) values ranged from 21.2% to 29.9%, all of which are below the species delimitation thresholds. The results of polyphasic taxonomy clearly indicated that strain Odt1-20B<sup>T</sup> represents a novel species, proposed as <i>Streptomyces odontotermitis</i> sp. nov. with the type strain Odt1-20B<sup>T</sup> ( = NBRC 116112 <sup>T</sup> = TBRC 16156 <sup>T</sup>).</p>

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Streptomyces odontotermitis Odt1-20BT, an actinobacterium isolated from the fungus-growing Odontotermes formosanus

  • 期刊论文

  • 发表日期：

    2026年5月26日

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  • Khomsan Supong,
  • Nantawan Niemhom,
  • Chanwit Suriyachadkun,
  • Somboon Tanasupawat,
  • Pattama Pittayakhajonwut

摘要

A novel culturable insect-derived actinobacterium, designated strain Odt1-20BT, was isolated from Odontotermes formosanus. The cells exhibited aerobic, Gram-stain-positive, and filamentous characteristics. Strain Odt1-20BT formed well-developed substrate and aerial mycelia, with straight spore chains borne on the aerial mycelium. Strain Odt1-20BT grew at 20–40 °C (optimum 30 °C), 0˗4% (w/v) NaCl, and pH 5˗10 (optimum 7−8). The l,l-diaminopimelic acid was determined in the whole-cell hydrolysate of strain Odt1-20BT. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine, whereas the detected major quinones were MK-9(H4), MK-9(H6), and MK-9(H8). Whole-cell sugars were galactose, glucose, mannose, ribose, and xylose. Summed feature C18:1 ω6c/ω7c, C16:0, anteiso-C15:0, and iso-C16:0 were the main fatty acids. The draft genome size was 10,091,787 nucleotides, with a G + C content of 71.5%. Comparative phylogenetic analysis based on 16S rRNA gene sequence demonstrated that strain Odt1-20BT belonged to the genus Streptomyces, and shared the highest similarity with S. davaonensis JCM 4913 T (98.55%). Furthermore, phylogenomic analysis showed that strain Odt1-20BT was clearly distinguished from the closely related Streptomyces species. The average nucleotide identity analyses based on BLAST (ANIb) and MUMmer (ANIm) between strain Odt1-20BT and its related species ranged below 87%, and the digital DNA-DNA hybridization (dDDH) values ranged from 21.2% to 29.9%, all of which are below the species delimitation thresholds. The results of polyphasic taxonomy clearly indicated that strain Odt1-20BT represents a novel species, proposed as Streptomyces odontotermitis sp. nov. with the type strain Odt1-20BT ( = NBRC 116112 T = TBRC 16156 T).