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<p>A novel <i>Streptomyces</i> species, designated strain DW26H14<sup>T</sup>, was isolated from duckweed (<i>Lemna aequinoctialis</i>) and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DW26H14<sup>T</sup> belonged to the genus <i>Streptomyces</i> and showed the highest similarity to <i>Streptomyces tremellae</i> Js-1<sup>T</sup> (98.8%) and <i>Streptomyces fuscigenes</i> JBL-20<sup>T</sup> (98.1%). The average nucleotide identity via BLAST (ANIb) and digital DNA-DNA hybridization (dDDH) values between strain DW26H14<sup>T</sup> and these closely related type strains ranged from 84.15–84.60% and 29.8–31.5% respectively, which were below the established thresholds for prokaryotic species delineation. Strain DW26H14<sup>T</sup> has a genome size of 8,003,460 bp with DNA G + C content of 72.18%. Chemotaxonomic analysis revealed that strain DW26H14<sup>T</sup> contained glucose, mannose, rhamnose, and ribose in its whole-cell hydrolysates. The major cellular fatty acids (&gt;10%) were C<sub>16:0</sub> and summed feature 8 (C<sub>18:1</sub> <i>ω7c</i>/C<sub>18:1</sub> <i>ω6c</i>). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositolmannosides, an unidentified aminolipid, and five unidentified phospholipids. The major menaquinones were MK-9(H<sub>4</sub>) and MK-9(H<sub>6</sub>). Based on the results of a polyphasic taxonomic analysis, strain DW26H14<sup>T</sup> represents a novel species within the genus <i>Streptomyces</i>, for which the name <i>Streptomyces lemnae</i> sp. nov. is proposed. The type strain is DW26H14<sup>T</sup> (=TBRC 17042<sup>T</sup> = NBRC 116115<sup>T</sup>).</p>

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Streptomyces lemnae sp. nov., a novel actinomycete isolated from Lemna aequinoctialis

  • 期刊论文

  • 发表日期：

    2026年2月27日

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  • Peeraya Boonchu,
  • Waranya Butdee,
  • Chadarat Na Phatthalung,
  • Chanita Boonmak,
  • Chanwit Suriyachadkun,
  • Kannika Duangmal

摘要

A novel Streptomyces species, designated strain DW26H14T, was isolated from duckweed (Lemna aequinoctialis) and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DW26H14T belonged to the genus Streptomyces and showed the highest similarity to Streptomyces tremellae Js-1T (98.8%) and Streptomyces fuscigenes JBL-20T (98.1%). The average nucleotide identity via BLAST (ANIb) and digital DNA-DNA hybridization (dDDH) values between strain DW26H14T and these closely related type strains ranged from 84.15–84.60% and 29.8–31.5% respectively, which were below the established thresholds for prokaryotic species delineation. Strain DW26H14T has a genome size of 8,003,460 bp with DNA G + C content of 72.18%. Chemotaxonomic analysis revealed that strain DW26H14T contained glucose, mannose, rhamnose, and ribose in its whole-cell hydrolysates. The major cellular fatty acids (>10%) were C16:0 and summed feature 8 (C18:1 ω7c/C18:1 ω6c). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositolmannosides, an unidentified aminolipid, and five unidentified phospholipids. The major menaquinones were MK-9(H4) and MK-9(H6). Based on the results of a polyphasic taxonomic analysis, strain DW26H14T represents a novel species within the genus Streptomyces, for which the name Streptomyces lemnae sp. nov. is proposed. The type strain is DW26H14T (=TBRC 17042T = NBRC 116115T).