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<p>A novel actinobacterium strain cg36<sup>T</sup>, from the rhizosphere of <i>Cyclosorus parasiticus</i>, was subjected to a polyphasic taxonomic study. 16S rRNA gene sequence analysis showed that strain cg36<sup>T</sup> shared the highest 16S rRNA gene sequence similarity to <i>Streptomyces lavendofoliae</i> NBRC 12882<sup>T</sup>. Phylogenetic analysis of 16S rRNA gene sequence indicated that strain cg36<sup>T</sup> formed a distinct branch within the genus <i>Streptomyces</i>. Phylogenetic analysis of five housekeeping gene and whole genome sequences showed that strain cg36<sup>T</sup> was closely related to <i>Streptomyces crystallinus</i> JCM 5067<sup>T</sup> and <i>Streptomyces noboritoensis</i> JCM 4557<sup>T</sup>. But, overall genome related index (OGRI) and multilocus sequence analysis (MLSA) confirmed that strain cg36<sup>T</sup> was clearly different from them. A comparison of differential features among strain cg36<sup>T</sup>, <i>S. crystallinus</i> CGMCC 4.1600<sup>T</sup> and <i>S. noboritoensis</i> CGMCC 4.1457<sup>T</sup> provided, at least to some extent, some useful information for strain cg36<sup>T</sup> as an independent species. In addition, OGRI values and MLSA evolutionary distance between strain cg36<sup>T</sup> and other strains, which had 16S rRNA gene sequence similarity of &gt;98.65% to strain cg36<sup>T</sup>, further demonstrated that strain cg36<sup>T</sup> was a new species. Whole-cell hydrolysates of strain cg36<sup>T</sup> contained <span>ll</span><i>-</i>diaminopimelic acid and whole-cell sugars contain glucose. The predominant cellular fatty acids (&gt;10%) were <i>anteiso-</i>C<sub>15:0</sub>, <i>iso-</i>C<sub>15:0</sub> and C<sub>16:0</sub>. The DNA G + C content of the genome sequence, consisting of 9,022,416 bp, was 72.5%. All these data indicated that strain cg36<sup>T</sup> represents a novel <i>Streptomyces</i> species, for which the name <i>Streptomyces cyclosori</i> sp. nov. is proposed. The type strain is strain cg36<sup>T</sup> (=MCCC 1K09286<sup>T</sup> = JCM 37520<sup>T</sup>).</p>

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Streptomyces cyclosori sp. nov., a novel actinobacterium from the rhizosphere soil of Cyclosorus parasiticus (L.) Farw

  • 期刊论文

  • 发表日期：

    2025年8月12日

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  • Rongjian Gao,
  • Yazi Chen,
  • Yan Xiao,
  • Jian Gao

摘要

A novel actinobacterium strain cg36T, from the rhizosphere of Cyclosorus parasiticus, was subjected to a polyphasic taxonomic study. 16S rRNA gene sequence analysis showed that strain cg36T shared the highest 16S rRNA gene sequence similarity to Streptomyces lavendofoliae NBRC 12882T. Phylogenetic analysis of 16S rRNA gene sequence indicated that strain cg36T formed a distinct branch within the genus Streptomyces. Phylogenetic analysis of five housekeeping gene and whole genome sequences showed that strain cg36T was closely related to Streptomyces crystallinus JCM 5067T and Streptomyces noboritoensis JCM 4557T. But, overall genome related index (OGRI) and multilocus sequence analysis (MLSA) confirmed that strain cg36T was clearly different from them. A comparison of differential features among strain cg36T, S. crystallinus CGMCC 4.1600T and S. noboritoensis CGMCC 4.1457T provided, at least to some extent, some useful information for strain cg36T as an independent species. In addition, OGRI values and MLSA evolutionary distance between strain cg36T and other strains, which had 16S rRNA gene sequence similarity of >98.65% to strain cg36T, further demonstrated that strain cg36T was a new species. Whole-cell hydrolysates of strain cg36T contained ll-diaminopimelic acid and whole-cell sugars contain glucose. The predominant cellular fatty acids (>10%) were anteiso-C15:0, iso-C15:0 and C16:0. The DNA G + C content of the genome sequence, consisting of 9,022,416 bp, was 72.5%. All these data indicated that strain cg36T represents a novel Streptomyces species, for which the name Streptomyces cyclosori sp. nov. is proposed. The type strain is strain cg36T (=MCCC 1K09286T = JCM 37520T).