Scholarlink

<p><i>Streptomyces</i> strain TP-A0890<sup>T</sup>, isolated from a soil sample, is a producer of FR-900452 and A-74863a. The taxonomic status was clarified by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to <i>Streptomyces coriariae</i>, with similarity of 99.7%. Strain TP-A0890<sup>T</sup> comprised <span>ll</span>-diaminopimelic acid, glutamic acid, glycine and alanine in its peptidoglycan. The predominant menaquinones were MK-9(H<sub>8</sub>) and MK-9(H<sub>6</sub>), and major fatty acids were <i>anteiso</i>-C<sub>17:0</sub>, <i>anteiso</i>-C<sub>15:0</sub>, <i>iso</i>-C<sub>16:0</sub> and <i>iso</i>-C<sub>17:0</sub>. The chemotaxonomic features matched those described for the genus <i>Streptomyces</i>. The genome size and G + C content were 8.72 Mb and 71.5%, respectively. The results of digital DNA-DNA hybridization along with differences in phenotypic characteristics between the strains suggested strain TP-A0890<sup>T</sup> to assign to a novel species, for which <i>Streptomyces maremycinicus</i> sp. nov. is proposed; the type strain is TP-A0890<sup>T</sup> ( = NBRC 110468<sup>T</sup>). We also show that <i>Streptomyces</i> strain B9173, a producer of FR-900452 and maremycins that was isolated from coastal sediment in Chile, belonged to <i>S. maremycinicus</i>. Twenty-two to 23 secondary metabolite-biosynthetic gene clusters (smBGCs) were present in the genomes of <i>S. maremycinicus</i> strains. Seventeen of them were conserved in the genome of <i>S. coriariae</i> CMB-FB<sup>T</sup> but the others were not.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Streptomyces maremycinicus sp. nov. and its secondary metabolite-biosynthetic gene clusters

  • 期刊论文

  • 发表日期：

    2025年7月9日

查看全文
  • Hisayuki Komaki,
  • Yasuhiro Igarashi,
  • Tomohiko Tamura

摘要

Streptomyces strain TP-A0890T, isolated from a soil sample, is a producer of FR-900452 and A-74863a. The taxonomic status was clarified by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Streptomyces coriariae, with similarity of 99.7%. Strain TP-A0890T comprised ll-diaminopimelic acid, glutamic acid, glycine and alanine in its peptidoglycan. The predominant menaquinones were MK-9(H8) and MK-9(H6), and major fatty acids were anteiso-C17:0, anteiso-C15:0, iso-C16:0 and iso-C17:0. The chemotaxonomic features matched those described for the genus Streptomyces. The genome size and G + C content were 8.72 Mb and 71.5%, respectively. The results of digital DNA-DNA hybridization along with differences in phenotypic characteristics between the strains suggested strain TP-A0890T to assign to a novel species, for which Streptomyces maremycinicus sp. nov. is proposed; the type strain is TP-A0890T ( = NBRC 110468T). We also show that Streptomyces strain B9173, a producer of FR-900452 and maremycins that was isolated from coastal sediment in Chile, belonged to S. maremycinicus. Twenty-two to 23 secondary metabolite-biosynthetic gene clusters (smBGCs) were present in the genomes of S. maremycinicus strains. Seventeen of them were conserved in the genome of S. coriariae CMB-FBT but the others were not.