Actinoplanes kirromycinicus sp. nov., isolated from soil

A novel actinomycete, designated as TPMA0078^T, was isolated from a soil sample collected in Shinjuku, Tokyo, Japan. 16S rRNA gene sequence analysis indicated that strain TPMA0078^T belongs to the genus Actinoplanes and is closely related to Actinoplanes regularis IFO 12514^T (99.86% 16S rRNA gene sequence similarity). The spores of strain TPMA0078^T were motile, and the sporangia were cylindrical. The diamino acids in the cell wall peptidoglycan of strain TPMA0078^T were meso-diaminopimelic acid and 3OH-meso-diaminopimelic acid. Whole-cell sugars were glucose and mannose, with galactose as a minor component. The major cellular fatty acids identified were iso-C_15:0, iso-C_16:0, and anteiso-C_17:0. The predominant menaquinone was MK-9(H₄), and the principal polar lipid was phosphatidylethanolamine. These chemotaxonomic properties of strain TPMA0078^T were consistent with those of Actinoplanes. Meanwhile, digital DNA–DNA hybridization and average nucleotide identity values showed low relatedness between strain TPMA0078^T and A. regularis NBRC 12514^T. Furthermore, several phenotypic properties of strain TPMA0078^T distinguished it from those of closely related species. Based on its genotypic and phenotypic characteristics, strain TPMA0078^T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes kirromycinicus sp. nov. is proposed. The type strain is TPMA0078^T (=NBRC 116422^T = TBRC 18262^T).