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<p>In this study, we developed two types of lattice-type β-cyclodextrin (β-CyD)-containing spherical hydrogels to immobilize phenol (PhOH)-degrading bacteria. One type, ENTG-<i>mix</i>-βCyD/HDI, consists of mixed-type spherical hydrogels containing β-CyD ring-bearing polymer microparticles embedded within the gel matrix. The other type, ENTG-<i>co-</i>PSβCyD, consists of copolymerized spherical hydrogels in which β-CyD-substituted monomers are copolymerized and crosslinked. The former features an aggregated distribution of β-CyD rings, whereas the latter exhibits a uniform distribution. Continuous PhOH degradation experiments revealed that both of the β-CyD-containing spherical hydrogel catalysts exhibited catalytic activity exceeding that of the ENTG spherical catalyst without β-CyD. Immobilized bacteria were distributed both on the surface and within the structure of the copolymerized carrier, whereas in the mixed carrier, many bacteria were dispersed throughout. Analysis of the PhOH-degrading flora revealed that <i>Pseudomonas putida</i> formed a niche in the copolymerized hydrogels, whereas <i>Sphingomonas</i> sp. formed a niche in the mixed hydrogels. Batch experiments using <i>p</i>-xylene instead of PhOH demonstrated that the degradation rates of the copolymerized and mixed gels were 2.4 times and 1.6 times greater than that of the ENTG gel, respectively. The copolymerized gel exhibited a faster <i>p</i>-xylene degradation rate due to the reactivity of <i>P. putida</i>.</p>

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Effects of β-Cyclodextrin Introduced by Different Methods on the Immobilized Phenol-Degrading Bacteria in Photocrosslinked Spherical Hydrogels

  • 期刊论文

  • 发表日期：

    2025年4月23日

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  • Hirohito Yamasaki,
  • Yasu-yuki Nagasawa,
  • Narumi Uchida,
  • Taiji Ito,
  • Kimitoshi Fukunaga

摘要

In this study, we developed two types of lattice-type β-cyclodextrin (β-CyD)-containing spherical hydrogels to immobilize phenol (PhOH)-degrading bacteria. One type, ENTG-mix-βCyD/HDI, consists of mixed-type spherical hydrogels containing β-CyD ring-bearing polymer microparticles embedded within the gel matrix. The other type, ENTG-co-PSβCyD, consists of copolymerized spherical hydrogels in which β-CyD-substituted monomers are copolymerized and crosslinked. The former features an aggregated distribution of β-CyD rings, whereas the latter exhibits a uniform distribution. Continuous PhOH degradation experiments revealed that both of the β-CyD-containing spherical hydrogel catalysts exhibited catalytic activity exceeding that of the ENTG spherical catalyst without β-CyD. Immobilized bacteria were distributed both on the surface and within the structure of the copolymerized carrier, whereas in the mixed carrier, many bacteria were dispersed throughout. Analysis of the PhOH-degrading flora revealed that Pseudomonas putida formed a niche in the copolymerized hydrogels, whereas Sphingomonas sp. formed a niche in the mixed hydrogels. Batch experiments using p-xylene instead of PhOH demonstrated that the degradation rates of the copolymerized and mixed gels were 2.4 times and 1.6 times greater than that of the ENTG gel, respectively. The copolymerized gel exhibited a faster p-xylene degradation rate due to the reactivity of P. putida.