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<p>Degumming with an aqueous alkaline solution, such as sodium carbonate (Na<sub>2</sub>CO<sub>3</sub>), is the initial step in purifying a silk fibroin (SF) aqueous solution from <i>Bombyx mori</i> silkworm cocoons. While essential for sericin removal, this process affects the molecular state of the SF, impacting factors such as the molecular chain length and weight distribution. In this study, we aimed to investigate the physical and biological properties of films prepared from different aqueous SF solutions obtained through degumming with Na<sub>2</sub>CO<sub>3</sub> at various concentrations, followed by water solubilization and dialysis. Higher concentrations of Na<sub>2</sub>CO<sub>3</sub> resulted in broader distributions of molecular weights in the purified SF aqueous solutions. An SF film containing a greater proportion of low-molecular-weight chains (below 100 kDa) exhibited a faster degradation rate and lower fibroblast adhesion compared with a film containing predominantly high-molecular-weight chains. In a rat abdominal adhesion model, the former inhibited postoperative adhesion, whereas the latter promoted adhesion at wound sites. These results highlight the importance of the initial degumming process in regulating the functionality of resulting SF products for medical applications.</p>

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The effect of degumming conditions on the functionality of silk fibroin films

  • 期刊论文

  • 发表日期：

    2025年2月21日

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  • Masaya Harada,
  • Wei Xu,
  • Yusuke Kambe,
  • Tsunenori Kameda,
  • Shoji Nagaoka,
  • Makoto Sasaki,
  • Takuro Niidome

摘要

Degumming with an aqueous alkaline solution, such as sodium carbonate (Na2CO3), is the initial step in purifying a silk fibroin (SF) aqueous solution from Bombyx mori silkworm cocoons. While essential for sericin removal, this process affects the molecular state of the SF, impacting factors such as the molecular chain length and weight distribution. In this study, we aimed to investigate the physical and biological properties of films prepared from different aqueous SF solutions obtained through degumming with Na2CO3 at various concentrations, followed by water solubilization and dialysis. Higher concentrations of Na2CO3 resulted in broader distributions of molecular weights in the purified SF aqueous solutions. An SF film containing a greater proportion of low-molecular-weight chains (below 100 kDa) exhibited a faster degradation rate and lower fibroblast adhesion compared with a film containing predominantly high-molecular-weight chains. In a rat abdominal adhesion model, the former inhibited postoperative adhesion, whereas the latter promoted adhesion at wound sites. These results highlight the importance of the initial degumming process in regulating the functionality of resulting SF products for medical applications.