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<p>Colorectal cancer (CRC), including both microsatellite instability (MSI) and microsatellite stability (MSS) subtypes, frequently exhibits intrinsic resistance to immunotherapy. However, the spatial tumor microenvironment (TME) and its role in distinguishing immunotherapy responders from non-responders remain poorly understood. In this study, spatial multiomics, including imaging mass cytometry (<i>n</i> = 50 in-house), spatial proteomics (<i>n</i> = 50 in-house), and spatial transcriptomics (<i>n</i> = 9 in-house), were employed to elucidate the spatial TME of metastatic CRC (mCRC) patients receiving immunotherapy. These methodologies were integrated with single-cell RNA sequencing (scRNA-seq), bulk RNA-seq, and bulk proteomics for comprehensive analysis and validation. A spatial immune atlas containing 314,774 cells was constructed. We found that C1QC<sup>+</sup> resident tissue macrophages (RTMs) were more abundant in responders regardless of microsatellite status. Co-localization of C1QC<sup>+</sup> RTMs with CD4<sup>+</sup> T cells was observed in responders, and MHC-II expression facilitated their interaction. In contrast, cancer-associated fibroblasts inhibited this interaction in non-responders. Moreover, whole genome screening identified key genes involved in antigen presentation in C1QC<sup>+</sup> RTMs. Hence, our study highlights the importance of spatial immune mapping in revealing the complex spatial topology of CRC and corresponding immunotherapy response.</p>

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Spatially resolved C1QC+ macrophage-CD4+ T cell niche in colorectal cancer microenvironment: implications for immunotherapy response

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Hangyu Zhang,
  • Libing Hong,
  • Zhen Dong,
  • Shan Xin,
  • Bo Lin,
  • Jinlin Cheng,
  • Weihong Tian,
  • Bin Li,
  • Jing Wang,
  • Xiaoyan Liu,
  • Chuan Liu,
  • Yuzhi Jin,
  • Yanzhi Feng,
  • Ge Su,
  • Xuqi Sun,
  • Qiqi Liu,
  • Xiaomeng Dai,
  • Yang Gao,
  • Zhou Tong,
  • Lulu Liu,
  • Xudong Zhu,
  • Yi Zheng,
  • Peng Zhao,
  • Tiannan Guo,
  • Weijia Fang,
  • Xuanwen Bao

摘要

Colorectal cancer (CRC), including both microsatellite instability (MSI) and microsatellite stability (MSS) subtypes, frequently exhibits intrinsic resistance to immunotherapy. However, the spatial tumor microenvironment (TME) and its role in distinguishing immunotherapy responders from non-responders remain poorly understood. In this study, spatial multiomics, including imaging mass cytometry (n = 50 in-house), spatial proteomics (n = 50 in-house), and spatial transcriptomics (n = 9 in-house), were employed to elucidate the spatial TME of metastatic CRC (mCRC) patients receiving immunotherapy. These methodologies were integrated with single-cell RNA sequencing (scRNA-seq), bulk RNA-seq, and bulk proteomics for comprehensive analysis and validation. A spatial immune atlas containing 314,774 cells was constructed. We found that C1QC+ resident tissue macrophages (RTMs) were more abundant in responders regardless of microsatellite status. Co-localization of C1QC+ RTMs with CD4+ T cells was observed in responders, and MHC-II expression facilitated their interaction. In contrast, cancer-associated fibroblasts inhibited this interaction in non-responders. Moreover, whole genome screening identified key genes involved in antigen presentation in C1QC+ RTMs. Hence, our study highlights the importance of spatial immune mapping in revealing the complex spatial topology of CRC and corresponding immunotherapy response.