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<p>This work evaluated the action of hsa_circ_0000520 in breast cancer (BC) progression through miR-542-3p/TMBIM6 axis. Clinical specimens were harvested from BC patients, in which hsa_circ_0000520 expression was determined. BT-549 cells were taken for transfection to intervene gene expression correspondingly, after which cellular proliferative, invasive, migratory, and apoptotic activities were monitored. As well, under different treatment, the changes in relevant indicators were observed. A tumor model was established in mice to examine the action of hsa_circ_0000520. Mechanistic analysis using RIP and lufiferase reporter assay were conducted to verify the interaction between miR-542-3p and hsa_circ_0000520 or TMBIM6. hsa_circ_0000520 was elevated in its expression in BC tissues. hsa_circ_0000520 knockdown exerted an anti-tumor property to fight against cellular malignant phenotypes in vitro, as well as tumor development in vivo. hsa_circ_0000520, through an absorption effect, regulated miR-542-3p, thereby targeting TMBIM6. miR-542-3p upregulation or TMBIM6 downregulation counter-balanced the pro-tumor effects of hsa_circ_0000520 overexpression. hsa_circ_0000520 promotes BC proliferation and metastasis through miR-542-3p-targeted TMBIM6.</p>

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The role of hsa_circ_0000520 in breast cancer progression: insights into the miR-542-3p/TMBIM6 regulatory axis

  • 期刊论文

  • 发表日期：

    2025年12月18日

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  • Feng Wang,
  • RenCheng Sun,
  • BaoKai Wang,
  • GengBao Qu

摘要

This work evaluated the action of hsa_circ_0000520 in breast cancer (BC) progression through miR-542-3p/TMBIM6 axis. Clinical specimens were harvested from BC patients, in which hsa_circ_0000520 expression was determined. BT-549 cells were taken for transfection to intervene gene expression correspondingly, after which cellular proliferative, invasive, migratory, and apoptotic activities were monitored. As well, under different treatment, the changes in relevant indicators were observed. A tumor model was established in mice to examine the action of hsa_circ_0000520. Mechanistic analysis using RIP and lufiferase reporter assay were conducted to verify the interaction between miR-542-3p and hsa_circ_0000520 or TMBIM6. hsa_circ_0000520 was elevated in its expression in BC tissues. hsa_circ_0000520 knockdown exerted an anti-tumor property to fight against cellular malignant phenotypes in vitro, as well as tumor development in vivo. hsa_circ_0000520, through an absorption effect, regulated miR-542-3p, thereby targeting TMBIM6. miR-542-3p upregulation or TMBIM6 downregulation counter-balanced the pro-tumor effects of hsa_circ_0000520 overexpression. hsa_circ_0000520 promotes BC proliferation and metastasis through miR-542-3p-targeted TMBIM6.