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Tumor-associated neutrophils suppress CD8+ T cell immunity in urothelial bladder carcinoma through the COX-2/PGE2/IDO1 Axis

  • 期刊论文

  • 发表日期：

    2024年1月17日

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  • Yi Ouyang,
  • Wenlong Zhong,
  • Peiqi Xu,
  • Bo Wang,
  • Lin Zhang,
  • Meng Yang,
  • Junyu Chen,
  • Hong Li,
  • Sheng Li,
  • Xiang Chen,
  • Longhao Xu,
  • Ziwei Ou,
  • Daqin Wu,
  • Yi Lin,
  • Chunhui Wang,
  • Jian Huang,
  • Tianxin Lin

摘要

Background

Many urothelial bladder carcinoma (UBC) patients don’t respond to immune checkpoint blockade (ICB) therapy, possibly due to tumor-associated neutrophils (TANs) suppressing lymphocyte immune response.

Methods

We conducted a meta-analysis on the predictive value of neutrophil-lymphocyte ratio (NLR) in ICB response and investigated TANs’ role in UBC. We used RNA-sequencing, HALO spatial analysis, single-cell RNA-sequencing, and flow cytometry to study the impacts of TANs and prostaglandin E2 (PGE2) on IDO1 expression. Animal experiments evaluated celecoxib’s efficacy in targeting PGE2 synthesis.

Results

Our analysis showed that higher TAN infiltration predicted worse outcomes in UBC patients receiving ICB therapy. Our research revealed that TANs promote IDO1 expression in cancer cells, resulting in immunosuppression. We also found that PGE2 synthesized by COX-2 in neutrophils played a key role in upregulating IDO1 in cancer cells. Animal experiments showed that targeting PGE2 synthesis in neutrophils with celecoxib enhanced the efficacy of ICB treatment.

Conclusions

TAN-secreted PGE2 upregulates IDO1, dampening T cell function in UBC. Celecoxib targeting of PGE2 synthesis represents a promising approach to enhance ICB efficacy in UBC.