Scholarlink

<p>Advancements in screening technologies employing small organisms have enabled deep profiling of compounds in vivo. However, current strategies for phenotyping of behaving animals, such as zebrafish, typically involve tedious manipulations. Here, we develop and validate a fully automated in vivo screening system (AISS) that integrates microfluidic technology and computer-vision-based control methods to enable rapid evaluation of biological responses of non-anesthetized zebrafish to molecular gradients. Via precise fluidic control, the AISS allows automatic loading, encapsulation, transportation and immobilization of single-larva in droplets for multi-organ imaging and chemical gradients generation inaccessible in previous systems. Using this platform, we examine the cardiac sensitivity of an antipsychotic drug with multiple concentration gradients, and reveal dramatic diversity and complexity in the accurate chemical regulation of cardiac functions in vivo. This proposed system expands the arsenal of tools available for in vivo screening and facilitates comprehensive profiling of pharmaceuticals.</p><p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fully automated in vivo screening system for multi-organ imaging and pharmaceutical evaluation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月27日

查看全文
  • Junhan Duan,
  • Guanming Lin,
  • Kangjian Jiao,
  • Xiaohui Hong,
  • Xudong Lin

摘要

Advancements in screening technologies employing small organisms have enabled deep profiling of compounds in vivo. However, current strategies for phenotyping of behaving animals, such as zebrafish, typically involve tedious manipulations. Here, we develop and validate a fully automated in vivo screening system (AISS) that integrates microfluidic technology and computer-vision-based control methods to enable rapid evaluation of biological responses of non-anesthetized zebrafish to molecular gradients. Via precise fluidic control, the AISS allows automatic loading, encapsulation, transportation and immobilization of single-larva in droplets for multi-organ imaging and chemical gradients generation inaccessible in previous systems. Using this platform, we examine the cardiac sensitivity of an antipsychotic drug with multiple concentration gradients, and reveal dramatic diversity and complexity in the accurate chemical regulation of cardiac functions in vivo. This proposed system expands the arsenal of tools available for in vivo screening and facilitates comprehensive profiling of pharmaceuticals.