Scholarlink

<p>Acid attack on stone cultural heritage is intrinsically linked to microbially driven nitrogen and sulfur cycling, yet the precise acid-producing metabolic machinery remains elusive. By integrating amplicon sequencing, DNA-stable isotope probing (DNA-SIP) and metagenomics, this study unveils a novel synergistic mechanism. A predominance of Candidatus <i>Nitrocosmicus</i> (AOA) and <i>Nitrosospira</i> (NOB) was found on the stone. Following <sup>15</sup>N incubation, active nitrification was confirmed by increased <i>amoA</i> and <i>nxrB</i> gene abundance and the production of <sup>15</sup>NO<sub>2</sub>⁻ and <sup>15</sup>NO<sub>3</sub>⁻. Furthermore, elevated <i>norB</i> and <i>nosZ</i> gene levels, alongside <sup>15</sup>NO<sub>2</sub>⁻ and <sup>15</sup>NO<sub>3</sub>⁻ depletion, confirmed an active denitrification. Concurrently, Dissimilatory Nitrate Reduction to Ammonium (DNRA) supplied the substrate by ammonia-oxidization, driving an internal nitrogen cycle. Crucially, metagenomics revealed coupling between denitrification and sulfur oxidation, demonstrating a synergistic cycle that accelerates acid production and salt damage. These findings establish a probable link between coupled nitrification-denitrification-sulfur oxidation and stone decay, providing a new conceptual framework for developing targeted biostatic strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synergistic nitrogen-sulfur metabolism driving biodeterioration revealed by metagenomic and DNA-SIP analyses at the Chen Cihong residence

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月11日

查看全文
  • Xueji Liang,
  • Xuping Gao,
  • Chaoyu Xie,
  • Tao Peng,
  • Ji-Dong Gu,
  • Zhong Hu,
  • Shanshan Meng

摘要

Acid attack on stone cultural heritage is intrinsically linked to microbially driven nitrogen and sulfur cycling, yet the precise acid-producing metabolic machinery remains elusive. By integrating amplicon sequencing, DNA-stable isotope probing (DNA-SIP) and metagenomics, this study unveils a novel synergistic mechanism. A predominance of Candidatus Nitrocosmicus (AOA) and Nitrosospira (NOB) was found on the stone. Following 15N incubation, active nitrification was confirmed by increased amoA and nxrB gene abundance and the production of 15NO2⁻ and 15NO3⁻. Furthermore, elevated norB and nosZ gene levels, alongside 15NO2⁻ and 15NO3⁻ depletion, confirmed an active denitrification. Concurrently, Dissimilatory Nitrate Reduction to Ammonium (DNRA) supplied the substrate by ammonia-oxidization, driving an internal nitrogen cycle. Crucially, metagenomics revealed coupling between denitrification and sulfur oxidation, demonstrating a synergistic cycle that accelerates acid production and salt damage. These findings establish a probable link between coupled nitrification-denitrification-sulfur oxidation and stone decay, providing a new conceptual framework for developing targeted biostatic strategies.