Scholarlink

<p>Parchment is a writing surface derived from animal skins. While sequencing the mitochondrial genome has been used to identify the parchment animal source, non-destructive sampling methods are pivotal because of their unique and irreplaceable nature. In this study, four different parchments were utilized to evaluate three non-destructive sampling methods: brushing, gecko tape, and forensic fibre lifts. For all methods, the impact of a 30-second eraser pre-cleaning to remove surface contaminants was assessed. After sample collection, total DNA was isolated, the mitochondrial genome enriched and sequenced using Illumina chemistry, and sequencing reads processed through a bioinformatics pipeline. Across all four documents, brushing with an eraser pre-cleaning was the optimal method, with an average of 98% of the mitochondrial genome recovered. Regardless of sampling method, collection after a 30-second eraser pre-cleaning resulted in higher source species DNA sequences. Non-destructive sampling will preserve documents with historical significance while allowing for genetic analysis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluating non-destructive sampling methods of parchment for genomic sequencing

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月5日

查看全文
  • Lindsey D. Diaz,
  • Melissa Scheible,
  • Timothy L. Stinson,
  • Isabella Livingston,
  • Matthew Breen,
  • Kelly A. Meiklejohn

摘要

Parchment is a writing surface derived from animal skins. While sequencing the mitochondrial genome has been used to identify the parchment animal source, non-destructive sampling methods are pivotal because of their unique and irreplaceable nature. In this study, four different parchments were utilized to evaluate three non-destructive sampling methods: brushing, gecko tape, and forensic fibre lifts. For all methods, the impact of a 30-second eraser pre-cleaning to remove surface contaminants was assessed. After sample collection, total DNA was isolated, the mitochondrial genome enriched and sequenced using Illumina chemistry, and sequencing reads processed through a bioinformatics pipeline. Across all four documents, brushing with an eraser pre-cleaning was the optimal method, with an average of 98% of the mitochondrial genome recovered. Regardless of sampling method, collection after a 30-second eraser pre-cleaning resulted in higher source species DNA sequences. Non-destructive sampling will preserve documents with historical significance while allowing for genetic analysis.