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<p><i>Rhododendron dauricum</i> essential oil (RDEO) has the effects of relieving cough, and expelling phlegm. The yield of RDEO obtained through traditional hydrodistillation (HD) is low. In this work, we used enzyme-assisted hydrodistillation (EAHD) and enzymatic hydrolysis caused severe damage to the cell structure of <i>Rhododendron dauricum.</i> The optimal extraction conditions for extracting RDEO using EAHD were obtained through response surface methodology with three factors and three levels. The optimum yield of RDEO of 2.163 ± 0.050&#xa0;mL/kg was obtained under the above mentioned conditions. Compared with the traditional HD method, the increase is 44.2%. The effects of enzymatic hydrolysis time and hydrodistillation time on the yield of essential oil were analyzed by the first and second order kinetic equations. It provides direction and scientific guidance for industrial scale production of RDEO. Under optimized conditions, 34 main components were detected. In addition, RDEO was used to measure its inhibitory effect on <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. The results indicate that, RDEO has good antibacterial activity, and its concentration is positively correlated with antibacterial activity. This work enriches the extraction technology of essential oils and provides direction for the research of using bioactive substances for antibacterial purposes.</p>

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Rhododendron dauricum essential oil obtained by enzyme-assisted hydrodistillation for antibacterial application

  • 期刊论文

  • 发表日期：

    2026年1月30日

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  • Nan An,
  • Gaofeng Zhang,
  • Lin Lu,
  • Haoyang Chen,
  • Liuke Zhang,
  • Gaolei Xi,
  • Xu Zhao,
  • Xinran Zhang,
  • Shuai Yu,
  • Tao Jia,
  • Xiuhua Zhao,
  • Lei Yang

摘要

Rhododendron dauricum essential oil (RDEO) has the effects of relieving cough, and expelling phlegm. The yield of RDEO obtained through traditional hydrodistillation (HD) is low. In this work, we used enzyme-assisted hydrodistillation (EAHD) and enzymatic hydrolysis caused severe damage to the cell structure of Rhododendron dauricum. The optimal extraction conditions for extracting RDEO using EAHD were obtained through response surface methodology with three factors and three levels. The optimum yield of RDEO of 2.163 ± 0.050 mL/kg was obtained under the above mentioned conditions. Compared with the traditional HD method, the increase is 44.2%. The effects of enzymatic hydrolysis time and hydrodistillation time on the yield of essential oil were analyzed by the first and second order kinetic equations. It provides direction and scientific guidance for industrial scale production of RDEO. Under optimized conditions, 34 main components were detected. In addition, RDEO was used to measure its inhibitory effect on Escherichia coli and Staphylococcus aureus. The results indicate that, RDEO has good antibacterial activity, and its concentration is positively correlated with antibacterial activity. This work enriches the extraction technology of essential oils and provides direction for the research of using bioactive substances for antibacterial purposes.