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<p>Cryopreservation represents an effective strategy for the long-term conservation of plant genetic resources. In this study, we investigated the genetic and physiological stability of cryopreserved plantlets and first-generation progeny of two virus-free potato (<i>Solanum tuberosum</i>) cultivars, Arinda and Santo. Plantlets were encapsulated and cryopreserved using ultra-low temperature storage. Post-recovery, key physiological parameters—including soluble protein, carbohydrate, and chlorophyll content—were assessed. Genetic stability was evaluated through polymerase chain reaction (PCR) amplification using 15 simple sequence repeat (SSR) markers. No significant differences (<i>p</i> &lt; 0.05) were detected in physiological traits between cryopreserved samples and non-cryopreserved in vitro controls. Furthermore, SSR analysis revealed no detectable polymorphisms, indicating 100% genetic stability across treatments. These findings confirm that encapsulation-based cryopreservation reliably maintains both physiological integrity and genetic fidelity in potato plantlets and their progeny. This approach offers a robust and sustainable method for preserving potato germplasm for future breeding and conservation programs.</p>

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Genetic and physiological stability in cryopreserved potato plantlets and their progeny via encapsulation techniques

  • 期刊论文

  • 发表日期：

    2026年2月5日

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  • Seyed Ali Mirbabaie,
  • Morteza Sam Daliri,
  • Reza Zarghami,
  • Amir Abbas Mousavi,
  • Ali Eftekhari

摘要

Cryopreservation represents an effective strategy for the long-term conservation of plant genetic resources. In this study, we investigated the genetic and physiological stability of cryopreserved plantlets and first-generation progeny of two virus-free potato (Solanum tuberosum) cultivars, Arinda and Santo. Plantlets were encapsulated and cryopreserved using ultra-low temperature storage. Post-recovery, key physiological parameters—including soluble protein, carbohydrate, and chlorophyll content—were assessed. Genetic stability was evaluated through polymerase chain reaction (PCR) amplification using 15 simple sequence repeat (SSR) markers. No significant differences (p < 0.05) were detected in physiological traits between cryopreserved samples and non-cryopreserved in vitro controls. Furthermore, SSR analysis revealed no detectable polymorphisms, indicating 100% genetic stability across treatments. These findings confirm that encapsulation-based cryopreservation reliably maintains both physiological integrity and genetic fidelity in potato plantlets and their progeny. This approach offers a robust and sustainable method for preserving potato germplasm for future breeding and conservation programs.