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<p>Carbon dots (CDs) have emerged as a new branch of research with many applications along with bio-applications. This research focuses on synthesizing CDs using pyrolysis technique. The average particle size of these fluorescent CDs was about 4.70&#xa0;nm which was confirmed by high-resolution transmission electron microscopy (HR-TEM). For the functional group detection, Fourier Transform Infrared Spectroscopy (FTIR) was used. The efficacy of CDs was checked on in vitro multiplication of the protocorms of <i>Cymbidium finlaysonianum</i> Lindl in Murashige and Skoog(1962) medium and its supplementation with growth regulators (NAA and BAP; 1&#xa0;mg L<sup>−1</sup>) and CDs (1–5 µL). The protocorms multiplied profusely in MS fortified with BAP and CDs. Up to 10 regenerants developed per explant. Auxin combined with the optimal dose of CDs (5 µL) favoured the multiplication of protocorm-like bodies(&#xa0;PLBs).</p>

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Role of carbon dots in the multiplication of the Germplasm of Cymbidium finlaysoniaum

  • 期刊论文

  • 发表日期：

    2026年1月28日

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  • Priyal Kaushik,
  • Gurmeet Kaur,
  • Saranjeet Kaur,
  • Somesh Malik,
  • Harkirat Kaur,
  • Imran Hasan

摘要

Carbon dots (CDs) have emerged as a new branch of research with many applications along with bio-applications. This research focuses on synthesizing CDs using pyrolysis technique. The average particle size of these fluorescent CDs was about 4.70 nm which was confirmed by high-resolution transmission electron microscopy (HR-TEM). For the functional group detection, Fourier Transform Infrared Spectroscopy (FTIR) was used. The efficacy of CDs was checked on in vitro multiplication of the protocorms of Cymbidium finlaysonianum Lindl in Murashige and Skoog(1962) medium and its supplementation with growth regulators (NAA and BAP; 1 mg L−1) and CDs (1–5 µL). The protocorms multiplied profusely in MS fortified with BAP and CDs. Up to 10 regenerants developed per explant. Auxin combined with the optimal dose of CDs (5 µL) favoured the multiplication of protocorm-like bodies( PLBs).