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<p>This study investigated the protective effects of a newly synthesized curcumin derivative compound against sodium arsenate toxicity in human fibroblast cells (CCD-1064Sk). The compound 4-((1E,6E)-7-(4-hydroxy,3-methoxyphenyl)-3,5-dioxohepta-1,6-dien-1-yl)-2-methoxyphenylpropionate (3) was synthesized from the reaction of curcumin (1) with propanoyl chloride (2) in the presence of acetone and sodium carbonate. We examined cell viability in the presence of sodium arsenate (0–500&#xa0;µM) and curcumin compound (0–200&#xa0;µg/mL). Cell death mechanisms were assessed using acridine orange/ethidium bromide and DAPI staining techniques, while expression levels of <i>ACTB</i>, <i>CASPASE-9</i>, and <i>BAX</i> genes were analyzed via real-time quantitative PCR. Results showed that 50&#xa0;µM sodium arsenate killed 48.5% of fibroblast cells within 24&#xa0;h. Cells treated with 200&#xa0;µg/mL curcumin compound exhibited 59% viability after 48&#xa0;h. Notably, <i>CASPASE-9</i> was overexpressed in sodium arsenate-treated cells but downregulated with curcumin compound. <i>BAX</i> expression decreased with curcumin compound treatment, indicating that the newly synthesized molecule may have protective effects against sodium arsenate toxicity.</p>

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A Novel Curcumin Compound Activity Against Sodium Arsenate Toxicity in Human Fibroblast Cell Line

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月12日

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  • Aksana Kavaleuskaya,
  • Nazlihan Dilan Elitas,
  • Burak Kilinc,
  • Cigdem Sezer Zhmurov,
  • Funda Ozkok,
  • Nihal Onul,
  • Tunc Catal

摘要

This study investigated the protective effects of a newly synthesized curcumin derivative compound against sodium arsenate toxicity in human fibroblast cells (CCD-1064Sk). The compound 4-((1E,6E)-7-(4-hydroxy,3-methoxyphenyl)-3,5-dioxohepta-1,6-dien-1-yl)-2-methoxyphenylpropionate (3) was synthesized from the reaction of curcumin (1) with propanoyl chloride (2) in the presence of acetone and sodium carbonate. We examined cell viability in the presence of sodium arsenate (0–500 µM) and curcumin compound (0–200 µg/mL). Cell death mechanisms were assessed using acridine orange/ethidium bromide and DAPI staining techniques, while expression levels of ACTB, CASPASE-9, and BAX genes were analyzed via real-time quantitative PCR. Results showed that 50 µM sodium arsenate killed 48.5% of fibroblast cells within 24 h. Cells treated with 200 µg/mL curcumin compound exhibited 59% viability after 48 h. Notably, CASPASE-9 was overexpressed in sodium arsenate-treated cells but downregulated with curcumin compound. BAX expression decreased with curcumin compound treatment, indicating that the newly synthesized molecule may have protective effects against sodium arsenate toxicity.