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<p>Glomalin-Related Soil Proteins (GRSP) are generally reported to originate from mycorrhizae, but there are suggestions that they may originate from various soil microorganisms. Biofertilizers aim to reduce the application of inorganic fertilizers, thereby improving soil health and positively influencing plant productivity. The objective of this study was to characterize GRSP in cultivated soil bioinoculated with a microbial consortium (MC) of the bacterial strains ME01 (<i>Rhizobium tropici</i>) + Leu2A (<i>Bradyrhizobium japonicum</i>). These strains were selected for their plant growth-promoting effects on <i>Allium cepa</i> L. under conditions of inorganic fertilization and water deficit. An onion crop was established with normal irrigation (NIr) (100% ETc), water deficit (WD) (67% ETc), and different fertilization treatments with MC (MC with 0%, 50%, and 100% NPK) in a semi-arid region of the Venezuelan Andes (San Juan de Lagunillas, Mérida state). The protein extract was purified using an extractant (NaOH/H<sub>2</sub>O), quantified using the universal Bradford method, and the proteins were visualized by SDS-PAGE. The results were analyzed using ANOVA. Significant differences were found in the number of proteins between treatments, suggesting that MC is part of the microorganisms that can produce GRSP, contributing to the field of soil microbiology. Intensive tillage did not affect the GRSP content. It is recommended to use GRSP quantification as an indicator of soil health and include bioinoculants in agricultural soils for sustainable agriculture.</p>

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Characterization of glomalin-related proteins in a bioinoculated soil in the presence of inorganic fertilizers and water deficit

  • 期刊论文

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  • 发表日期：

    2025年10月30日

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  • Erika Lorena Blanco,
  • Robert Márquez,
  • Danny Camargo,
  • Jorge Almarza,
  • Fermín Rada,
  • Jorge Paolini

摘要

Glomalin-Related Soil Proteins (GRSP) are generally reported to originate from mycorrhizae, but there are suggestions that they may originate from various soil microorganisms. Biofertilizers aim to reduce the application of inorganic fertilizers, thereby improving soil health and positively influencing plant productivity. The objective of this study was to characterize GRSP in cultivated soil bioinoculated with a microbial consortium (MC) of the bacterial strains ME01 (Rhizobium tropici) + Leu2A (Bradyrhizobium japonicum). These strains were selected for their plant growth-promoting effects on Allium cepa L. under conditions of inorganic fertilization and water deficit. An onion crop was established with normal irrigation (NIr) (100% ETc), water deficit (WD) (67% ETc), and different fertilization treatments with MC (MC with 0%, 50%, and 100% NPK) in a semi-arid region of the Venezuelan Andes (San Juan de Lagunillas, Mérida state). The protein extract was purified using an extractant (NaOH/H2O), quantified using the universal Bradford method, and the proteins were visualized by SDS-PAGE. The results were analyzed using ANOVA. Significant differences were found in the number of proteins between treatments, suggesting that MC is part of the microorganisms that can produce GRSP, contributing to the field of soil microbiology. Intensive tillage did not affect the GRSP content. It is recommended to use GRSP quantification as an indicator of soil health and include bioinoculants in agricultural soils for sustainable agriculture.