Scholarlink

<p>A chitosan-modified nanocomposite of reduced graphene oxide and nanoceria (CH-(rGO@CeO<sub>2</sub>) NC) was synthesised using co-precipitation method to develop an electrochemical biosensor for the detection of the antibiotic gentamicin (GEN). Various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) and contact angle (CA) were employed for morphological and chemical characterization. Differential pulse voltammetry (DPV) demonstrated excellent electrochemical activity of CH-(rGO@CeO<sub>2</sub>) NC, making it an ideal transducer material for developing a highly sensitive electrochemical immunosensor. This immunosensor utilized monoclonal antibodies against gentamicin (anti-GEN) that was covalently bound to the CH-(rGO@CeO<sub>2</sub>) NC modified screen-printed electrode (SPE). Protein kinase A (PKA) was used to regulate immunologic interactions, while BSA was applied to block nonspecific binding sites on the anti-GEN/CH-(rGO@CeO<sub>2</sub>)/SPE surface. The DPV data for the BSA-anti-GEN/CH-(rGO@CeO<sub>2</sub>)/SPE immunoelectrode in GEN detection showed a satisfactory linear detection range (1&#xa0;pM-100&#xa0;µM), with a limit of detection (LOD) of 0.570&#xa0;pM and a sensitivity of 2.88 µA pM<sup>−1</sup>&#xa0;cm<sup>−2</sup>. The analysis exhibited a strong linear relationship with an R<sup>2</sup> value of 0.973. This CH-(rGO@CeO<sub>2</sub>) electrochemical immunosensor demonstrated excellent potential for detecting GEN in spiked milk and tap water samples.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Electrochemical immunosensor development with chitosan modified reduced graphene oxide-nanoceria nanocomposite for gentamicin detection

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月5日

查看全文
  • Jayendra Kumar Himanshu,
  • G. B. V. S. Lakshmi,
  • S. Z. H. Hashmi,
  • Ahmed Kareem Hussein Alatafi,
  • Akhilesh Kumar Singh,
  • Pratima R. Solanki

摘要

A chitosan-modified nanocomposite of reduced graphene oxide and nanoceria (CH-(rGO@CeO2) NC) was synthesised using co-precipitation method to develop an electrochemical biosensor for the detection of the antibiotic gentamicin (GEN). Various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) and contact angle (CA) were employed for morphological and chemical characterization. Differential pulse voltammetry (DPV) demonstrated excellent electrochemical activity of CH-(rGO@CeO2) NC, making it an ideal transducer material for developing a highly sensitive electrochemical immunosensor. This immunosensor utilized monoclonal antibodies against gentamicin (anti-GEN) that was covalently bound to the CH-(rGO@CeO2) NC modified screen-printed electrode (SPE). Protein kinase A (PKA) was used to regulate immunologic interactions, while BSA was applied to block nonspecific binding sites on the anti-GEN/CH-(rGO@CeO2)/SPE surface. The DPV data for the BSA-anti-GEN/CH-(rGO@CeO2)/SPE immunoelectrode in GEN detection showed a satisfactory linear detection range (1 pM-100 µM), with a limit of detection (LOD) of 0.570 pM and a sensitivity of 2.88 µA pM−1 cm−2. The analysis exhibited a strong linear relationship with an R2 value of 0.973. This CH-(rGO@CeO2) electrochemical immunosensor demonstrated excellent potential for detecting GEN in spiked milk and tap water samples.