<p>This study examines the bioremediation potential of <i>Azolla</i>, an aquatic fern, for managing nutrient levels in fish ponds, specifically nitrate and phosphate concentrations. In 2023, four fresh water fish ponds located at Sirakol in South 24 Parganas district, West Bengal (India), were selected to monitor seasonal variations in nutrient levels and bioaccumulation of nitrogen and phosphorus within <i>Azolla</i>. Sampling and analysis were conducted across three distinct seasonal phases, namely premonsoon, monsoon, and postmonsoon. Findings revealed that nutrient levels were highest during the monsoon, followed by a decrease in the postmonsoon period, with the lowest concentrations recorded in the premonsoon season. This seasonal trend was mirrored in the nitrogen and phosphorus bioaccumulation within <i>Azolla</i>, demonstrating the fern's dynamic capacity to absorb these nutrients as environmental concentrations fluctuate. Data analysis revealed significant positive correlations between the nitrate and phosphate concentrations in the pond water and the levels of nitrogen and phosphorus accumulated within <i>Azolla</i> tissues. The correlation values strongly support the role of <i>Azolla</i> as an effective bio-tool to upgrade the water quality, which naturally purifies pond water by absorbing excess nutrients that often originate from sources such as residual fish feed, fish excreta, and agricultural runoff from nearby fields. The findings underscore <i>Azolla</i>'s role as a sustainable option for nutrient management in fresh water fish pond. By naturally reducing nitrate and phosphate levels, <i>Azolla</i> not only improves water quality but also mitigates the ecological impact of nutrient pollution. This approach aligns with environmentally sustainable practices in aquaculture, addressing nutrient management through biological means rather than chemical interventions.</p>