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<p><i>Euphorbia hirta</i> Linn. is a medicinal plant found in the Philippines and known for its application in dengue management. <i>E. hirta</i> collected from different geographical locations have demonstrated variations in anti-thrombocytopenic activity in vivo<i>.</i> Hence, it is necessary to determine the intraspecific diversity of <i>E. hirta</i> to provide insights into observed bioactivity. For this study, thirty-nine (39) <i>E. hirta</i> plant specimens were collected from the three major Philippine island groups: Luzon (n = 24), Visayas (n = 10), Mindanao (n = 5). Species identity was confirmed through DNA barcoding using primers from <i>rbcL</i> and <i>matK</i> genes. Identification of intraspecies genetic variations was made through Sequence-Related Amplified Polymorphism (SRAP) analysis. Three (3) SRAP primer pairs produced 23 distinct and reproducible bands with a polymorphism average of 84.63%. The average values of the evaluation indices Nei’s genetic diversity (H) and Shannon’s diversity index (I), and the Polymorphic Information Content (PIC) of the major Philippine geographical groups were 0.3376 ± 0.024, 0.480 ± 0.034, and 0.397, respectively. A low genetic differentiation (F<sub>st</sub> = 0.042) was also found between the <i>E. hirta</i> accessions. UPGMA clustering based on the presence or absence of these bands grouped the plant samples into three clusters. Geographical distance did not play a role in the clustering. Two gene loci were also identified to have potential use as markers for species identification. The study has generated DNA fingerprints of SRAP markers and attempted to identify <i>E. hirta</i> genetic variants. This may be adapted as a standard method in the molecular characterization of medicinally important plant samples.</p>

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Assessment of genetic diversity of Euphorbia hirta Linn in the Philippines using sequence-related amplified polymorphism (SRAP) markers

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月21日

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  • Patrick Gabriel G. Moreno,
  • Patrick R. Relacion,
  • Francisco M. Heralde III

摘要

Euphorbia hirta Linn. is a medicinal plant found in the Philippines and known for its application in dengue management. E. hirta collected from different geographical locations have demonstrated variations in anti-thrombocytopenic activity in vivo. Hence, it is necessary to determine the intraspecific diversity of E. hirta to provide insights into observed bioactivity. For this study, thirty-nine (39) E. hirta plant specimens were collected from the three major Philippine island groups: Luzon (n = 24), Visayas (n = 10), Mindanao (n = 5). Species identity was confirmed through DNA barcoding using primers from rbcL and matK genes. Identification of intraspecies genetic variations was made through Sequence-Related Amplified Polymorphism (SRAP) analysis. Three (3) SRAP primer pairs produced 23 distinct and reproducible bands with a polymorphism average of 84.63%. The average values of the evaluation indices Nei’s genetic diversity (H) and Shannon’s diversity index (I), and the Polymorphic Information Content (PIC) of the major Philippine geographical groups were 0.3376 ± 0.024, 0.480 ± 0.034, and 0.397, respectively. A low genetic differentiation (Fst = 0.042) was also found between the E. hirta accessions. UPGMA clustering based on the presence or absence of these bands grouped the plant samples into three clusters. Geographical distance did not play a role in the clustering. Two gene loci were also identified to have potential use as markers for species identification. The study has generated DNA fingerprints of SRAP markers and attempted to identify E. hirta genetic variants. This may be adapted as a standard method in the molecular characterization of medicinally important plant samples.