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<p>A micropropagation protocol for BARI Lilium-1 (<i>Lilium longiflorum</i>) was developed using stem nodes as explants to enable large-scale cultivation in Bangladesh. Explants were surface sterilized with 0.2% Ridomil gold (20&#xa0;min), followed by detergent solution (10&#xa0;min) and finally with commercial bleach (60% chlorox). Sterilized explants were cultured on Murashige and Skoog (MS) medium containing varying concentrations and combinations of BAP, NAA, IAA and IBA for shoot bud initiation with multiple shooting. Subsequently, for root induction, different concentrations of IBA and NAA were examined individually. The highest percentage of explant-producing shoot buds (93%), the greatest number of bulblets (31.66) and the longest bulblets (11.49&#xa0;cm) were achieved with MS medium containing 1&#xa0;mg/L each of IAA and IBA. Optimal rooting, with 6.65 roots per bulblet within the shortest time (10.55&#xa0;days), was achieved in MS medium supplemented with 0.5&#xa0;mg/L NAA. Most of the bulblets were easily acclimatized and showed 100% survival during primary hardening in a soil and coco peat (1:2) mixture, demonstrating the robustness of this protocol. This study highlights a reproducible and efficient tissue culture method for mass propagation of BARI Lilium-1 within a shorter period of time, which will greatly benefit the cut flower industry in Bangladesh.</p>

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In vitro mass propagation protocol for BARI lilium-1 adapted in Bangladesh

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月6日

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  • Mahmuda Khatun,
  • Mustare Ahamed Madobe,
  • Shyam Chandra Halder,
  • Md. Abdullah Yousuf Akhond

摘要

A micropropagation protocol for BARI Lilium-1 (Lilium longiflorum) was developed using stem nodes as explants to enable large-scale cultivation in Bangladesh. Explants were surface sterilized with 0.2% Ridomil gold (20 min), followed by detergent solution (10 min) and finally with commercial bleach (60% chlorox). Sterilized explants were cultured on Murashige and Skoog (MS) medium containing varying concentrations and combinations of BAP, NAA, IAA and IBA for shoot bud initiation with multiple shooting. Subsequently, for root induction, different concentrations of IBA and NAA were examined individually. The highest percentage of explant-producing shoot buds (93%), the greatest number of bulblets (31.66) and the longest bulblets (11.49 cm) were achieved with MS medium containing 1 mg/L each of IAA and IBA. Optimal rooting, with 6.65 roots per bulblet within the shortest time (10.55 days), was achieved in MS medium supplemented with 0.5 mg/L NAA. Most of the bulblets were easily acclimatized and showed 100% survival during primary hardening in a soil and coco peat (1:2) mixture, demonstrating the robustness of this protocol. This study highlights a reproducible and efficient tissue culture method for mass propagation of BARI Lilium-1 within a shorter period of time, which will greatly benefit the cut flower industry in Bangladesh.