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<p>Monosaccharide transporters (MSTs) are important plant glucose transporters that play roles in carbon allocation, growth, development, and stress regulation. However, research on MST regulatory genes in soybeans remains unexplored. In this study, we identified 110 MST-like candidate genes in the <i>Glycine max</i> L. (soybean) genome. The MST genes were distributed throughout 20 chromosomes, with many undergoing segmental duplication. The majority of MST group proteins exhibit significant preservation in Arabidopsis and soybean, and phylogenetic analysis reveals seven main categories. The promoters of the <i>GmMST</i> genes comprise cis-acting elements associated with plant responses to abscisic acid, auxin signaling, methyl jasmonate, low temperatures, and abiotic stresses. The RNA-seq data demonstrated diverse expression levels of MST genes across various tissues or organs, categorizing them into 11 primary clusters. The co-expression study of the network complex indicated that the <i>GmMST</i> genes encode proteins that interact with many essential genes associated with gibberellin, which are pivotal in seed and stamen development. A qPCR study validated the expression patterns of seven MST genes with exposure to copper (Cu) and cadmium (Cd) stress. Certain MST genes were notably stimulated by both Cu and Cd stress treatments, suggesting their involvement in defense responsiveness. Therefore, we provide a comprehensive analysis of the MST genes in soybeans and emphasize their substantial role in plant development and tolerance to metal ion stressors.</p>

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Comprehensive in silico, characterization and expression profiling of MST genes in soybean (Glycine max) reveal their roles in development and metal ion responses

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月20日

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  • Intikhab Alam,
  • Hantao Ge,
  • Hakim Manghwar,
  • Naveed ur Rehman,
  • Hui-Cong Wang,
  • Fang Qiao

摘要

Monosaccharide transporters (MSTs) are important plant glucose transporters that play roles in carbon allocation, growth, development, and stress regulation. However, research on MST regulatory genes in soybeans remains unexplored. In this study, we identified 110 MST-like candidate genes in the Glycine max L. (soybean) genome. The MST genes were distributed throughout 20 chromosomes, with many undergoing segmental duplication. The majority of MST group proteins exhibit significant preservation in Arabidopsis and soybean, and phylogenetic analysis reveals seven main categories. The promoters of the GmMST genes comprise cis-acting elements associated with plant responses to abscisic acid, auxin signaling, methyl jasmonate, low temperatures, and abiotic stresses. The RNA-seq data demonstrated diverse expression levels of MST genes across various tissues or organs, categorizing them into 11 primary clusters. The co-expression study of the network complex indicated that the GmMST genes encode proteins that interact with many essential genes associated with gibberellin, which are pivotal in seed and stamen development. A qPCR study validated the expression patterns of seven MST genes with exposure to copper (Cu) and cadmium (Cd) stress. Certain MST genes were notably stimulated by both Cu and Cd stress treatments, suggesting their involvement in defense responsiveness. Therefore, we provide a comprehensive analysis of the MST genes in soybeans and emphasize their substantial role in plant development and tolerance to metal ion stressors.