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<p>Outbreaks of diseases associated with food/food pathogen-borne illness have been advancing recently. With limited options available to completely eradicate these pathogens, further research is essential to understand their mechanisms and develop effective control strategies. <i>Cucumis callosus</i> is a medicinal plant that has shown tremendous therapeutic application for non-communicable diseases. Thus, the local and improved AHK-119 variety of this plant were evaluated for its antimicrobial potential using in-vitro assays [zone of inhibition &amp; minimum inhibitory concentration (MIC)] and in-silico validation (Molecular docking, simulation and ADMET profiling). The in-vitro result indicated that the extracts of this plant has potentially increased antimicrobial activity with increased concentration. As per the findings, the MIC and ZOI against <i>Aspergillus niger</i>,<i> Streptococcus aureus</i>, and <i>Pseudomonas aeruginosa</i> was 50&#xa0;µg and 8 ± 0&#xa0;mm, 500&#xa0;µg and 6.66 ± 0.57&#xa0;mm, and 50&#xa0;µg and 6 ± 0&#xa0;mm for improved AHK-119 variety and 50&#xa0;µg and 8 ± 0&#xa0;mm, 1000&#xa0;µg and 7 ± 0&#xa0;mm, and 50&#xa0;µg and 6 ± 0&#xa0;mm for local variety, respectively. Further, the results of in-silico validation revealed that molecular docking analysis of bioactive compounds from <i>Cucumis callosus</i> has significant interactions with major pathogens, including <i>Streptococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aspergillus niger</i>. Among the compounds, stigmasterol exhibited the highest binding affinity against <i>Pseudomonas aeruginosa</i> (− 7.0&#xa0;kcal/mol) and showed strong interaction with <i>Streptococcus aureus</i> (− 8.3&#xa0;kcal/mol). In contrast, stigmastan-3-ol demonstrated the highest binding affinity against <i>Aspergillus niger</i> (− 6.5&#xa0;kcal/mol). Thus, the findings ensured the antimicrobial potential of both the local and improved varieties of <i>C. callosus</i> against food pathogens and indicated its potential applications for industrial and health applications, particularly as a basis for the development of functional food products.</p>

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Comparative analysis of antimicrobial property of local and improved varieties of Cucumis callosus against food pathogens

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月19日

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  • Deepika,
  • Raushan Kumar Jha,
  • Sujata Negi,
  • Surbhi Panwar,
  • Daisy Musahary,
  • Surender Singh,
  • Ashwani Kumar,
  • Anita Kumari

摘要

Outbreaks of diseases associated with food/food pathogen-borne illness have been advancing recently. With limited options available to completely eradicate these pathogens, further research is essential to understand their mechanisms and develop effective control strategies. Cucumis callosus is a medicinal plant that has shown tremendous therapeutic application for non-communicable diseases. Thus, the local and improved AHK-119 variety of this plant were evaluated for its antimicrobial potential using in-vitro assays [zone of inhibition & minimum inhibitory concentration (MIC)] and in-silico validation (Molecular docking, simulation and ADMET profiling). The in-vitro result indicated that the extracts of this plant has potentially increased antimicrobial activity with increased concentration. As per the findings, the MIC and ZOI against Aspergillus niger, Streptococcus aureus, and Pseudomonas aeruginosa was 50 µg and 8 ± 0 mm, 500 µg and 6.66 ± 0.57 mm, and 50 µg and 6 ± 0 mm for improved AHK-119 variety and 50 µg and 8 ± 0 mm, 1000 µg and 7 ± 0 mm, and 50 µg and 6 ± 0 mm for local variety, respectively. Further, the results of in-silico validation revealed that molecular docking analysis of bioactive compounds from Cucumis callosus has significant interactions with major pathogens, including Streptococcus aureus, Pseudomonas aeruginosa, and Aspergillus niger. Among the compounds, stigmasterol exhibited the highest binding affinity against Pseudomonas aeruginosa (− 7.0 kcal/mol) and showed strong interaction with Streptococcus aureus (− 8.3 kcal/mol). In contrast, stigmastan-3-ol demonstrated the highest binding affinity against Aspergillus niger (− 6.5 kcal/mol). Thus, the findings ensured the antimicrobial potential of both the local and improved varieties of C. callosus against food pathogens and indicated its potential applications for industrial and health applications, particularly as a basis for the development of functional food products.