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Background <p>Bacterial and helminthic evolution has resulted in a shocking development of antibiotic and anthelminthic resistance. Medicinal plants provide an encouraging source to develop new antibacterial and anthelmintic agents<i>. Rubus ellipticus</i> has been employed traditionally for medicinal purposes, yet its ability against microbial resistance remains to be unraveled.</p> Methods <p>The present work assessed the antioxidant, antibacterial, and anthelmintic activities of leaf extracts of <i>R. ellipticus</i>. Antioxidant activity was measured using DPPH, antibacterial activity was done with the serial dilution technique, and anthelmintic activity was performed by using Indian earthworms. Molecular docking experiments were also carried out by utilizing Maestro 12.7 (Schrodinger) in order to screen the interaction between phytoconstituents with bacterial as well as helminthic targets.</p> Results <p>The methanol extract exhibited the highest antioxidant activity (%RSA = 90.95 ± 0.47%) and was the most potent antibacterial, with a minimum inhibitory concentration (MIC) value of 12.5&#xa0;µg/mL against <i>Bacillus subtilis</i> and <i>Escherichia coli</i>. Paralysis and death time of earthworms were significantly reduced by methanol and acetone extracts at 20&#xa0;mg/mL (paralysis: 12 ± 1.00&#xa0;min, death: 15.33 ± 1.20&#xa0;min). The molecular docking outcomes showed that kaempferol, ellagic acid, gallic acid, rutin, anthocyanin, and quercetin had good binding affinities towards the target proteins.</p> Conclusion <p>The results indicated that <i>R. ellipticus</i> is a potential natural source of bioactive compounds with antibacterial and anthelmintic activity. Additional research, including in vivo and mechanistic studies, is suggested to confirm its therapeutic uses.</p>

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In vitro and in silico anthelmintic and antibacterial activity of Rubus ellipticus leaf extracts

  • 期刊论文

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  • 发表日期：

    2025年6月27日

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  • Deepak Kumar,
  • Adarsh Kumar,
  • Ankit Kumar Singh,
  • Harshwardhan Singh,
  • Pradeep Kumar

摘要

Background

Bacterial and helminthic evolution has resulted in a shocking development of antibiotic and anthelminthic resistance. Medicinal plants provide an encouraging source to develop new antibacterial and anthelmintic agents. Rubus ellipticus has been employed traditionally for medicinal purposes, yet its ability against microbial resistance remains to be unraveled.

Methods

The present work assessed the antioxidant, antibacterial, and anthelmintic activities of leaf extracts of R. ellipticus. Antioxidant activity was measured using DPPH, antibacterial activity was done with the serial dilution technique, and anthelmintic activity was performed by using Indian earthworms. Molecular docking experiments were also carried out by utilizing Maestro 12.7 (Schrodinger) in order to screen the interaction between phytoconstituents with bacterial as well as helminthic targets.

Results

The methanol extract exhibited the highest antioxidant activity (%RSA = 90.95 ± 0.47%) and was the most potent antibacterial, with a minimum inhibitory concentration (MIC) value of 12.5 µg/mL against Bacillus subtilis and Escherichia coli. Paralysis and death time of earthworms were significantly reduced by methanol and acetone extracts at 20 mg/mL (paralysis: 12 ± 1.00 min, death: 15.33 ± 1.20 min). The molecular docking outcomes showed that kaempferol, ellagic acid, gallic acid, rutin, anthocyanin, and quercetin had good binding affinities towards the target proteins.

Conclusion

The results indicated that R. ellipticus is a potential natural source of bioactive compounds with antibacterial and anthelmintic activity. Additional research, including in vivo and mechanistic studies, is suggested to confirm its therapeutic uses.