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<p>Pesticide residue in fruits and vegetables poses significant risks to human beings, birds, and aquatic creatures as it causes cancer, liver, kidney, and heart-related diseases. Traditional pesticide residue analysis using chromatographic techniques is time-consuming and expensive. Biologically synthesized metal nanoparticles offer a promising solution for pesticide sensing due to their non-toxic, cost-effective, reliable, and environment-friendly properties. In this research, L-cysteine-modified silver nanoparticles (AgNPs) were synthesized using <i>Parmelia sulcata</i> to detect malathion. The synthesized AgNPs were characterized using UV–visible spectroscopy, XRD, SEM, and EDX. The change in color and absorption peak at 427&#xa0;nm in the UV–visible spectrophotometer indicates the formation of nanoparticles. The XRD and SEM revealed the crystal size of the nanoparticles to be 24.7&#xa0;nm with a face-centered cubic (FCC) structure. The EDX confirmed the presence of metallic silver in the nanoparticles. Modified L-cysteine-AgNPs showed increased optical absorbance from 0.1278 to 0.1581 due to shell formation around NPs through coordination between AgNPs and the thiol group. Modified AgNPs were used as a colorimetric sensor to perceive malathion at different concentrations. Color change from strong yellow to light yellow on increasing the concentration of pesticide and the absorbance at 427&#xa0;nm gradually increase with the decrease in concentration of the malathion. Thus L-cys-AgNPs can be used as a qualitative method for the detection of malathion.</p>

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Biosynthesis of L-cysteine modified silver nanoparticle using Parmelia sulcata T and applied to detect malathion

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月17日

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  • Achyut Adhikari,
  • Prajwal Acharya,
  • Prabina Shrestha,
  • Aarati Ray,
  • Kapil Aryal,
  • Shraddha Kandel

摘要

Pesticide residue in fruits and vegetables poses significant risks to human beings, birds, and aquatic creatures as it causes cancer, liver, kidney, and heart-related diseases. Traditional pesticide residue analysis using chromatographic techniques is time-consuming and expensive. Biologically synthesized metal nanoparticles offer a promising solution for pesticide sensing due to their non-toxic, cost-effective, reliable, and environment-friendly properties. In this research, L-cysteine-modified silver nanoparticles (AgNPs) were synthesized using Parmelia sulcata to detect malathion. The synthesized AgNPs were characterized using UV–visible spectroscopy, XRD, SEM, and EDX. The change in color and absorption peak at 427 nm in the UV–visible spectrophotometer indicates the formation of nanoparticles. The XRD and SEM revealed the crystal size of the nanoparticles to be 24.7 nm with a face-centered cubic (FCC) structure. The EDX confirmed the presence of metallic silver in the nanoparticles. Modified L-cysteine-AgNPs showed increased optical absorbance from 0.1278 to 0.1581 due to shell formation around NPs through coordination between AgNPs and the thiol group. Modified AgNPs were used as a colorimetric sensor to perceive malathion at different concentrations. Color change from strong yellow to light yellow on increasing the concentration of pesticide and the absorbance at 427 nm gradually increase with the decrease in concentration of the malathion. Thus L-cys-AgNPs can be used as a qualitative method for the detection of malathion.