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<p>Hemorrhagic disease caused by bluetongue virus (BTV) significantly impacts Florida’s deer farming industry each year. In 2022, we investigated mortality events in white-tailed deer on two separate farms. Necropsies were performed on affected animals, and tissues were subjected to RT-qPCR screening and molecular diagnostics, confirming BTV infection. Virus isolation in C6/36 cell culture and whole-genome sequencing identified BTV serotype 5 (BTV-5) infection in both fawns—the first cases reported in Florida since 2003. Our analyses further reveal that key serotype-defining genome segments (2 and 6) remained conserved, while other segments exhibited evidence of reassortment with co-circulating BTV strains in the region. This suggests that BTV-5 may have persisted undetected at low levels, undergoing genetic exchange with co-circulating strains. These findings highlight the need for improved surveillance programs to monitor genetic changes and mitigate impacts on deer and other ruminants in Florida.</p>

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Genome characterization of two bluetongue virus serotype 5 strains isolated from white-tailed deer: first detected cases in Florida after 19 years

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月24日

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  • Pacharapong Khrongsee,
  • Pedro H. O. Viadanna,
  • Kristen N. Wilson,
  • Tracey L. Moquin,
  • Juan M. Campos Krauer,
  • John A. Lednicky,
  • Samantha M. Wisely,
  • Kuttichantran Subramaniam

摘要

Hemorrhagic disease caused by bluetongue virus (BTV) significantly impacts Florida’s deer farming industry each year. In 2022, we investigated mortality events in white-tailed deer on two separate farms. Necropsies were performed on affected animals, and tissues were subjected to RT-qPCR screening and molecular diagnostics, confirming BTV infection. Virus isolation in C6/36 cell culture and whole-genome sequencing identified BTV serotype 5 (BTV-5) infection in both fawns—the first cases reported in Florida since 2003. Our analyses further reveal that key serotype-defining genome segments (2 and 6) remained conserved, while other segments exhibited evidence of reassortment with co-circulating BTV strains in the region. This suggests that BTV-5 may have persisted undetected at low levels, undergoing genetic exchange with co-circulating strains. These findings highlight the need for improved surveillance programs to monitor genetic changes and mitigate impacts on deer and other ruminants in Florida.