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<p>A major worldwide issue contributing to the spread of antibiotic resistance genes is antibiotic contamination of environmental matrices. Although amoxicillin is frequently found in the environment, little is known about its bioremediation. In view of this, the current study focused on amoxicillin removal using <i>Burkholderia</i> sp. WA, an amoxicillin-resistant bacterium. The bacterium was isolated using a selective enrichment approach. Using amoxicillin as the sole carbon source in M9 minimal medium, the amoxicillin concentration was raised from 5&#xa0;mg/L to 50&#xa0;mg/L. The bacteria were characterized using biochemical tests, antibiotic tests, and molecular analysis. Strain WA exhibited yellowish-brown colonies on LB agar media. The bacteria were resistant to amikacin, ampicillin, ciprofloxacin, and chloramphenicol, along with amoxicillin. The minimum inhibitory concentration of the bacteria against amoxicillin was found to be 128&#xa0;µg/mL. The strain WA revealed 100% similarity to <i>Burkholderia cepacia</i> &amp; <i>Burkholderia</i> sp. strain TJ13. Further, the amoxicillin removal was seen using ultra-power liquid chromatography, and up to 69% of amoxicillin was eliminated from the M9 minimal medium. Our study is the first study to use <i>Burkholderia</i> sp., which shows the amoxicillin removal in M9 minimal media. This study further opens the avenue for the treatment of amoxicillin-contaminated water using bioremediation. This is a viable method for minimising antibiotic pollution in soil and water.</p>

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Characterization and application of Burkholderia sp. WA in mitigating amoxicillin pollution

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月20日

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  • Kushneet Kaur Sodhi,
  • Chandra Kant Singh,
  • Dileep Kumar Singh

摘要

A major worldwide issue contributing to the spread of antibiotic resistance genes is antibiotic contamination of environmental matrices. Although amoxicillin is frequently found in the environment, little is known about its bioremediation. In view of this, the current study focused on amoxicillin removal using Burkholderia sp. WA, an amoxicillin-resistant bacterium. The bacterium was isolated using a selective enrichment approach. Using amoxicillin as the sole carbon source in M9 minimal medium, the amoxicillin concentration was raised from 5 mg/L to 50 mg/L. The bacteria were characterized using biochemical tests, antibiotic tests, and molecular analysis. Strain WA exhibited yellowish-brown colonies on LB agar media. The bacteria were resistant to amikacin, ampicillin, ciprofloxacin, and chloramphenicol, along with amoxicillin. The minimum inhibitory concentration of the bacteria against amoxicillin was found to be 128 µg/mL. The strain WA revealed 100% similarity to Burkholderia cepacia & Burkholderia sp. strain TJ13. Further, the amoxicillin removal was seen using ultra-power liquid chromatography, and up to 69% of amoxicillin was eliminated from the M9 minimal medium. Our study is the first study to use Burkholderia sp., which shows the amoxicillin removal in M9 minimal media. This study further opens the avenue for the treatment of amoxicillin-contaminated water using bioremediation. This is a viable method for minimising antibiotic pollution in soil and water.