Out of tissue engineering systemic crisis by means of holographic 3D-photopolymerization

Tissue engineering, despite decades of focus on medical applications, has yielded minimal progress in this respect, drastically dissimilar to the avalanche of efforts undertaken. There’s no even a conceptual approach, besides recently sprung up “Linear Volumetric Printing”. Which, however, has not yet been tested even on biocompatible materials.

While existing technologies are limited to creating 3D structures that are either two orders of magnitude larger or three orders of magnitude slower to produce than required for medical-grade bioprinting.

This study proposes a novel approach to overcome these limitations and achieve the creation of fully functional medical grade tissue-engineered constructs.

We propose a multi-pronged approach: 1. Define Minimal Requirements: Establish the essential characteristics of bare minimum requirements for fully functional tissue-engineered constructs. 2. Pulsed Holographic Bioprinting: Develop a pulsed holographic submicron-scale photopolymerization system for generating intricate scaffolds (including vascular networks and stromal compartments), based on a mathematical hologram model; 3. Multi-Material Volumetric Printing: Develop a methodology for multi-material volumetric photopolymerization to create complex structures. 4. Cellular Delivery Systems: Design “service conductive dispersion-framework systems” to efficiently deliver cells and bioactive substrates throughout the bioengineered tissue strata, combined with wireframe pseudo-stroma; 5. Gas Transport Culture Medium: Develop a novel gas transport perfusion culture medium for optimal growth and maturation of bioengineered tissues/organs prior to implantation.

We believe that this combined approach will address existing limitations and pave the way for the creation of fully functional bioengineered tissues and organs for medical applications, offering some utterly fantastic prospects.